Description

N,N'-Trimethylenebis(Iodoacetamide) is a specialized bifunctional alkylating agent featuring two reactive iodoacetamide groups linked by a trimethylene spacer. This compound matters for its ability to act as a potent cross-linking reagent, primarily used to introduce stable thioether bonds by reacting with sulfhydryl (thiol) groups in proteins, peptides, and other biomolecules. It is essential for researchers and manufacturers in the life sciences sector who require precise molecular modification, protein conjugation, and the study of protein structure and function.

Application

• Protein Cross-linking and Conjugation: A key reagent for creating stable conjugates between proteins, antibodies, or peptides and other molecules like enzymes, fluorophores, or drugs.
• Bioconjugation Chemistry: Used to modify thiol-containing biomolecules for applications in diagnostics, targeted drug delivery, and biosensor development.
• Structural Biology Studies: Employed to probe protein folding, dynamics, and intermolecular interactions by introducing specific cross-links.
• Enzyme Inhibition Studies: Serves as an active site-directed irreversible inhibitor for thiol-dependent enzymes, aiding in mechanistic studies.
• Material Surface Functionalization: Used to tether biomolecules onto surfaces (e.g., gold, polymers) for creating bioactive coatings and lab-on-a-chip devices.
• Peptide Synthesis: Acts as a building block or modifying agent in the synthesis of complex peptides with specific reactive handles.

Basic Information

Product Name	N,N'-Trimethylenebis(Iodoacetamide)
CAS No.	57355-26-7
Molecular Formula	C7H10I2N2O2
Molecular Weight	423.97 g/mol
Synonyms	1,3-Bis(iodoacetamido)propane; N,N'-Propane-1,3-diylbis(2-iodoacetamide); 2-Iodo-N-[3-[(2-iodoacetyl)amino]propyl]acetamide; Trimethylenebis(iodoacetamide); Bis(iodoacetamido)propane; Iodoacetamide Trimethylene Dimer; IAA Crosslinker
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of N,N'-Trimethylenebis(Iodoacetamide) is characterized using advanced analytical techniques to confirm identity, purity, and consistency. Certificates of Analysis (COA) detailing specifications such as assay (HPLC), melting point, and impurity profiles are available upon request. We adhere to strict quality management protocols to support research and development requiring high-purity biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed under anhydrous conditions and away from direct light exposure to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.