Description

2-Fluorohistidine is a fluorinated derivative of the essential amino acid L-histidine, where a fluorine atom is strategically substituted, significantly altering its biochemical properties. This modification is crucial for probing enzyme mechanisms, particularly in histidine-dependent enzymes, and for studying protein structure-function relationships. It serves as a vital biochemical tool for researchers and developers in pharmaceutical discovery, proteomics, and advanced biochemical research, enabling detailed metabolic pathway analysis and the development of novel enzyme inhibitors.

Application

• Enzyme Mechanism Studies: Used as a mechanistic probe to investigate the role of histidine residues in enzyme active sites, particularly in hydrolytic enzymes like proteases and phosphatases.
• Pharmaceutical Research & Development: Serves as a key intermediate or building block in the design and synthesis of novel drug candidates targeting histidine-mediated biological pathways.
• Metabolic Pathway Analysis: Employed in biochemical assays to trace and understand metabolic processes involving histidine, including studies on fluorinated amino acid metabolism.
• Protein Engineering & Labeling: Utilized for site-specific incorporation into proteins via peptide synthesis or mutagenesis to study protein folding, stability, and interactions.
• Biochemical Reagent: Acts as a specialized substrate or inhibitor in high-throughput screening assays for drug discovery and diagnostic development.
• Academic & Institutional Research: Fundamental research tool in universities and institutes for studies in bioorganic chemistry, chemical biology, and medicinal chemistry.

Basic Information

Product Name	2-Fluorohistidine
CAS No.	57212-36-9
Molecular Formula	C6H9FN3O2
Molecular Weight	174.16 g/mol
Synonyms	2-Fluoro-L-histidine; (2S)-2-Amino-3-(2-fluoro-1H-imidazol-4-yl)propanoic acid; L-2-Fluorohistidine; 2-FH; 2-F-His; 2-Fluoroimidazole-4-alanine; 2-Fluorohistidine, L-; 2-Fluoro-1H-imidazole-4-propanoic acid, α-amino-, (S)-
EINECS	Contact for details

Quality Control

Our 2-Fluorohistidine is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis, and spectroscopic identification. A detailed Certificate of Analysis (COA) providing full specifications and test results is supplied with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Chiral Purity (HPLC)	≥ 98.0% (L-isomer)
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.