Description

Cyclo(Histidyl-Leucyl) is a synthetic cyclic dipeptide, also known as a diketopiperazine (DKP), formed from the amino acids L-Histidine and L-Leucine. This compound is of significant interest in pharmaceutical research and development due to its role as a potential bioactive scaffold and a degradation product or impurity in peptide-based drugs. It serves as a critical reference standard and building block for researchers in medicinal chemistry, biochemistry, and analytical laboratories focused on peptide stability and metabolism.

Application

• Pharmaceutical Reference Standard: Used for the identification, purity assessment, and quantification of related substances in peptide Active Pharmaceutical Ingredients (APIs) and finished drug products.
• Medicinal Chemistry Research: Acts as a core scaffold or intermediate for the synthesis of novel drug candidates targeting various biological pathways.
• Biochemical Studies: Employed in research to study enzyme inhibition, receptor binding, and the biological activity of cyclic dipeptides.
• Analytical Method Development: Serves as a critical component in developing and validating HPLC, LC-MS, and other chromatographic methods for peptide analysis.
• Peptide Stability Testing: Used to monitor and understand degradation pathways in therapeutic peptides during formulation and storage.

Basic Information

Product Name	Cyclo(Histidyl-Leucyl)
CAS No.	55253-69-5
Molecular Formula	C12H18N4O2
Molecular Weight	250.30 g/mol
Synonyms	Cyclo(His-Leu); Cyclo(L-histidyl-L-leucyl); L-Histidyl-L-leucine diketopiperazine; 3-(2-Methylpropyl)-6-(1H-imidazol-5-ylmethyl)-2,5-piperazinedione; Histidine-leucine diketopiperazine; c(His-Leu); DKP-His-Leu; Cyclo(histidine-leucine)
EINECS	Contact for details

Quality Control

Our Cyclo(Histidyl-Leucyl) is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis, and identity confirmation (IR, MS). We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or quality control standards. Specifications can be tailored to meet the requirements of pharmacopeial (e.g., USP) or internal methods.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.