Description

Tetramethylbenzidine (CAS NO 54827-17-7) is a highly sensitive chromogenic substrate widely recognized for its critical role in enzymatic detection systems. This compound is essential for producing a distinct blue colorimetric signal, enabling precise and reliable quantification in analytical assays. It is a fundamental reagent for research, diagnostic, and quality control laboratories requiring high-performance detection methods.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): Primary chromogenic substrate for horseradish peroxidase (HRP) in clinical diagnostics and biomedical research.
• Immunohistochemistry (IHC): Used for visualizing antigen localization in tissue sections with HRP-conjugated antibodies.
• Western Blotting: Employed as a detection reagent for HRP-labeled proteins on membranes.
• Biosensor Development: Integral component in the design of electrochemical and optical biosensors for pathogen or analyte detection.
• Water & Environmental Testing: Applied in test kits for the detection of peroxidases or peroxidase-like activity in environmental samples.
• Forensic Analysis: Utilized in presumptive tests for the detection of blood (catalase activity).
• Food Safety Testing: Used in enzymatic assays to detect specific contaminants or residues.

Basic Information

Product Name	Tetramethylbenzidine
CAS No.	54827-17-7
Molecular Formula	C16H20N2
Molecular Weight	240.35 g/mol
Synonyms	3,3',5,5'-Tetramethylbenzidine; TMB; TMB Free Base; 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine; 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl; NSC 406130; Benzidine, 3,3',5,5'-tetramethyl-
EINECS	259-364-6

Quality Control

Our Tetramethylbenzidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets the high standards required for sensitive diagnostic and research applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Melting Point	168 - 172 °C
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, ethanol; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.