Description

Gly-Ala-Ala-D-Ala-D-Ala is a synthetic pentapeptide sequence containing both L- and D-amino acid isomers. This specific stereochemistry is critical for its function as a substrate and inhibitor in enzymatic studies, particularly those involving bacterial cell wall biosynthesis. It is primarily utilized by research institutions and pharmaceutical companies engaged in antibiotic development and peptidoglycan research. The compound's defined structure provides a valuable tool for investigating enzyme specificity and resistance mechanisms.

Application

• Biochemical Research: Used as a specific substrate for studying the activity of enzymes like D-alanyl-D-alanine carboxypeptidases and transpeptidases involved in bacterial peptidoglycan synthesis.
• Antibiotic Development: Serves as a key intermediate or inhibitor in screening programs for novel antibiotics that target cell wall assembly, such as vancomycin analogs.
• Enzyme Kinetics & Inhibition Studies: Employed to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) for enzymes that recognize the D-Ala-D-Ala terminus.
• Microbiology Research: Applied in studies of bacterial resistance mechanisms, particularly vancomycin resistance, where modification of the D-Ala-D-Ala peptide target is crucial.
• Peptide Library Synthesis: Acts as a building block or a core sequence in the construction of specialized peptide libraries for high-throughput screening.
• Analytical Standard: Used as a reference standard in HPLC, LC-MS, or other analytical methods for quantifying related peptides or enzymatic products.

Basic Information

Product Name	Gly-Ala-Ala-D-Ala-D-Ala
CAS No.	53093-86-0
Molecular Formula	C16H27N5O7
Molecular Weight	401.42 g/mol
Synonyms	Glycyl-L-alanyl-L-alanyl-D-alanyl-D-alanine; G-A-A-D-A-D-A; H-Gly-Ala-Ala-D-Ala-D-Ala-OH; Pentapeptide G-A-A-D-A-D-A; D-Ala-D-Ala containing pentapeptide; Substrate for DD-carboxypeptidase; Vancomycin binding peptide sequence; N-(N-(N-(N-Glycyl-L-alanyl)-L-alanyl)-D-alanyl)-D-alanine
EINECS	Contact for details

Quality Control

Every batch of Gly-Ala-Ala-D-Ala-D-Ala is produced and analyzed under strict quality management protocols. Our products undergo rigorous quality testing, including multiple orthogonal analytical methods, to ensure identity, purity, and consistency for research applications. Certificates of Analysis (COA) detailing batch-specific results for purity, chiral integrity, and solvent residues are available upon request. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Chiral Purity (HPLC)	≥ 98.0% (D-isomer at positions 4 & 5)
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.