Description

Z-Gly-Gly-Leu-Pna is a synthetic peptide substrate containing a para-nitroaniline (Pna) chromogenic leaving group. This compound is specifically designed as a selective and sensitive substrate for the study and quantification of proteolytic enzyme activity. It is a critical reagent for researchers and manufacturers in the life sciences sector who require precise tools for enzyme kinetics, inhibitor screening, and diagnostic assay development. The product is supplied as a high-purity, well-characterized biochemical for reliable and reproducible results.

Application

• Enzyme Kinetics & Mechanism Studies: Used as a chromogenic substrate to investigate the catalytic activity, specificity, and inhibition profiles of proteases, particularly those cleaving after leucine residues.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research for the rapid screening of potential therapeutic enzyme inhibitors in drug discovery pipelines.
• Diagnostic Assay Development: Serves as a key component in the development of colorimetric or spectrophotometric clinical diagnostic tests for detecting specific enzyme levels.
• Biochemical Research: A fundamental tool for academic and industrial research into protease function, protein degradation pathways, and cellular signaling mechanisms.
• Quality Control in Biomanufacturing: Utilized to monitor and validate the activity of enzyme-based products or processes within controlled manufacturing environments.

Basic Information

Product Name	Z-Gly-Gly-Leu-Pna
CAS No.	53046-98-3
Molecular Formula	C22H27N5O7
Molecular Weight	473.48 g/mol
Synonyms	Z-Gly-Gly-Leu-pNA; Benzyloxycarbonyl-Glycyl-Glycyl-L-Leucine 4-Nitroanilide; Carbobenzoxy-Gly-Gly-Leu-pNA; N-CBZ-Gly-Gly-Leu-pNA; CBZ-Gly-Gly-Leu-4-nitroanilide; Substrate for Protease; Chromogenic Peptide Substrate; L-Leucine, N-[(phenylmethoxy)carbonyl]glycylglycyl-, 4-nitroanilide
EINECS	Contact for details

Quality Control

Our Z-Gly-Gly-Leu-Pna is manufactured under stringent quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee the material meets the rigorous standards required for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.