Description

Suc-Ala-Ala-Ala-Pna is a specialized synthetic peptide substrate designed for enzymatic research and biochemical analysis. This compound is critical for studying protease activity, particularly that of chymotrypsin and related serine proteases, enabling precise kinetic measurements and inhibitor screening. It serves as an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, high-purity biochemical reagents.

Application

• Protease Enzyme Assays: Primary substrate for the spectrophotometric determination of chymotrypsin, elastase, and subtilisin activity.
• Drug Discovery & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors.
• Biochemical Research: A key reagent for kinetic studies, enzyme mechanism elucidation, and substrate specificity profiling in academic and industrial R&D.
• Diagnostic Kit Manufacturing: Incorporated as a core component in enzymatic diagnostic kits for clinical and research use.
• Quality Control Testing: Employed in pharmaceutical QC labs to standardize and validate protease activity in enzyme preparations.

Basic Information

Product Name	Suc-Ala-Ala-Ala-Pna
CAS No.	52299-14-6
Molecular Formula	C₂₀H₂₇N₅O₈
Molecular Weight	465.46 g/mol
Synonyms	N-Succinyl-Ala-Ala-Ala-p-nitroanilide; Suc-AAA-pNA; Suc-Ala-Ala-Ala-pNA; N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide; Suc-(Ala)₃-pNA; Protease Substrate IV; Chymotrypsin Substrate Suc-Ala-Ala-Ala-pNA; N-Succinyl-tri-L-alanine p-nitroanilide
EINECS	Contact for details

Quality Control

Our Suc-Ala-Ala-Ala-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometric assays for functional performance. Certificates of Analysis (COA) with detailed chromatograms and full traceability are provided to ensure compliance with research and industrial standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	≥ 95% of reference standard in chymotrypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.