Description

2',3'-Di-o-(tert-Butyldimethylsilyl)-2'-Deoxycytidine is a key protected nucleoside derivative, specifically a silyl-protected form of deoxycytidine. This compound is critically important as a versatile synthetic intermediate in the production of oligonucleotides, antisense drugs, and other nucleotide-based therapeutics. It is primarily needed by research institutions, pharmaceutical R&D departments, and fine chemical manufacturers specializing in nucleic acid chemistry and prodrug development.

Application

• Oligonucleotide Synthesis: Serves as a crucial building block (phosphoramidite precursor) in solid-phase synthesis of DNA and RNA strands, where the tert-butyldimethylsilyl (TBDMS) groups protect the 2' and 3' hydroxyls.
• Antisense & siRNA Therapeutics: Used in the research and development of antisense oligonucleotides and small interfering RNA (siRNA) molecules for targeted gene silencing applications.
• Prodrug Development: Acts as an intermediate in the synthesis of nucleoside prodrugs, enhancing stability and bioavailability of active pharmaceutical ingredients (APIs).
• Chemical Biology Research: Employed as a tool compound for studying nucleic acid structure, function, and for labeling nucleotides.
• Pharmaceutical Intermediates: A key starting material for the synthesis of more complex, modified nucleosides with antiviral or anticancer activity.
• Diagnostic Reagent Manufacturing: Used in the production of labeled nucleotides for diagnostic assays and sequencing technologies.

Basic Information

Product Name	2',3'-Di-o-(tert-Butyldimethylsilyl)-2'-Deoxycytidine
CAS No.	51549-29-2
Molecular Formula	C24H47N3O4Si2
Molecular Weight	497.82 g/mol
Synonyms	2'-Deoxycytidine, 2',3'-bis-O-[(1,1-dimethylethyl)dimethylsilyl]-; 2',3'-Bis-O-(tert-butyldimethylsilyl)-2'-deoxycytidine; 2',3'-Di-O-TBDMS-deoxycytidine; N4-Benzoyl-2',3'-di-O-(tert-butyldimethylsilyl)-2'-deoxycytidine (protected variant); dC(TBDMS)2; TBDMS-protected deoxycytidine; 1-[(2R,4S,5R)-4-[(tert-Butyldimethylsilyl)oxy]-5-([(tert-butyldimethylsilyl)oxy]methyl)oxolan-2-yl]-4-aminopyrimidin-2-one
EINECS	Contact for details

Quality Control

Our 2',3'-Di-o-(tert-Butyldimethylsilyl)-2'-Deoxycytidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and LC-MS for identity verification, to ensure it meets the high standards required for pharmaceutical and research applications. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and purity. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.