Description

Hemoglobin Leiden is a stable variant of hemoglobin, a critical oxygen-transporting metalloprotein found in red blood cells. This specific variant is of significant interest in biochemical research and diagnostic development due to its altered oxygen-binding properties. It is primarily utilized by research institutions, diagnostic manufacturers, and pharmaceutical companies for studies in hematology, oxygen transport kinetics, and as a reference material in clinical assays.

Application

• Biochemical Research: Used as a model protein for studying hemoglobin structure-function relationships, oxygen affinity, and allosteric regulation.
• Diagnostic Reagent Manufacturing: Serves as a key component or calibrator in the production of in-vitro diagnostic (IVD) kits for blood gas analysis and hemoglobinopathy testing.
• Pharmaceutical Development: Employed in preclinical research for developing therapeutic agents targeting hemoglobin disorders or oxygen delivery systems.
• Clinical Reference Material: Acts as a stable control or standard in clinical laboratories for quality assurance of hemoglobin electrophoresis and chromatography tests.
• Educational & Teaching Tools: Supplied to academic institutions for advanced biochemistry and physiology laboratory courses.

Basic Information

Product Name	Hemoglobin Leiden
CAS No.	50926-06-2
Molecular Formula	C2952H4664N812O832S40Fe4
Molecular Weight	~64.5 kDa (Tetramer)
Synonyms	Hb Leiden; Hemoglobin variant Leiden; alpha2beta2(6 or 7) Glu->0; Deoxyhemoglobin Leiden; Oxyhemoglobin Leiden; Methemoglobin Leiden; Hemoglobin, Leiden variant
EINECS	Contact for details

Quality Control

Our Hemoglobin Leiden is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and functional integrity. Certificates of Analysis (COA) detailing specifications such as purity (by HPLC), spectral properties (UV-Vis), and functional assay are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Under these conditions, the product is typically stable for several years. Avoid repeated freeze-thaw cycles. The material is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (UV-Vis)	Conforms to reference spectrum
Purity (HPLC)	≥ 95% (Hemoglobin Leiden)
Assay (Functional)	≥ 90% of theoretical oxygen-binding capacity
Methemoglobin Content	< 5%
Protein Concentration	As specified per batch
Endotoxin	< 1.0 EU/mg (if for research use)
Solubility	Clear solution in buffer (e.g., PBS, pH 7.4)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.