Description

Boc-Ala-Ala-Pna CAS NO 50439-35-5 is a protected tripeptide derivative, specifically a chromogenic substrate designed for enzymatic studies. This compound is a critical reagent in biochemical research for the specific detection and quantification of protease activity. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for enzyme kinetics, inhibitor screening, and assay development.

Application

• Protease Activity Assays: As a chromogenic substrate for the specific detection and kinetic analysis of various proteolytic enzymes, including elastase and related serine proteases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: A fundamental tool for studying enzyme mechanisms, substrate specificity, and catalytic efficiency in academic and industrial R&D laboratories.
• Diagnostic Kit Development: Employed in the formulation of diagnostic reagents and kits for detecting protease levels associated with specific physiological or pathological conditions.
• Quality Control in Biomanufacturing: Used to monitor protease activity during the production and purification of biopharmaceuticals to ensure product consistency and purity.

Basic Information

Product Name	Boc-Ala-Ala-Pna
CAS No.	50439-35-5
Molecular Formula	C19H27N5O6
Molecular Weight	421.45 g/mol
Synonyms	Boc-Ala-Ala-pNA; N-(tert-Butoxycarbonyl)-L-alanyl-L-alanine 4-nitroanilide; tert-Butyloxycarbonyl-Ala-Ala-p-nitroanilide; Boc-L-Ala-L-Ala-pNA; BOC-ALA-ALA-PNA; Substrate for Elastase; Nα-Boc-L-alanyl-L-alanine 4-nitroanilide; Elastase Substrate I
EINECS	Contact for details

Quality Control

Our Boc-Ala-Ala-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for identity and residual solvents. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with your research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.