Description

Ac-Ala-Ala-Phe-OMe is a synthetic tripeptide derivative, specifically an acetylated alanyl-alanyl-phenylalanine methyl ester. This compound serves as a crucial research-grade biochemical building block and enzyme substrate, particularly valuable for studying protease specificity and kinetics. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies engaged in peptide chemistry and enzymology.

Application

• Protease/Enzyme Substrate: Used as a specific chromogenic or fluorogenic substrate for studying the activity and inhibition of proteolytic enzymes like chymotrypsin and subtilisin.
• Biochemical Research: Serves as a key intermediate or model compound in peptide synthesis research and structure-activity relationship (SAR) studies.
• Pharmaceutical Development: Employed in the discovery and screening of novel protease inhibitors with potential therapeutic applications.
• Academic Studies: Utilized in university laboratories for teaching advanced concepts in enzymology, peptide chemistry, and biochemical kinetics.
• Quality Control Reagent: Acts as a reference standard for calibrating analytical instruments and validating methods in peptide analysis.

Basic Information

Product Name	Ac-Ala-Ala-Phe-OMe
CAS No.	50395-42-1
Molecular Formula	C20H28N4O6
Molecular Weight	420.46 g/mol
Synonyms	Acetyl-Ala-Ala-Phe methyl ester; N-Acetyl-L-alanyl-L-alanyl-L-phenylalanine methyl ester; Ac-AAF-OMe; Ac-AA-Phe-OMe; Acetylalanylalanylphenylalanine Methyl Ester; L-Phenylalanine, N-acetyl-L-alanyl-L-alanyl-, methyl ester; AAF-pNA (related chromogenic substrate); Protease Substrate III (common research designation)
EINECS	Contact for details

Quality Control

Our Ac-Ala-Ala-Phe-OMe is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, chiral analysis, and identity confirmation (IR, MS). A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants or storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on anhydrous basis)
Optical Rotation	[α]D20: Specific range provided on COA
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.