Description

n-Formyl-Met-Leu-Phe is a synthetic formylated tripeptide widely recognized as a potent and specific chemoattractant for neutrophils and other phagocytic cells. This compound is a critical research tool for studying the formyl peptide receptor (FPR) signaling pathway, inflammation, and immune cell chemotaxis. It is essential for researchers and developers in the fields of immunology, pharmacology, and inflammatory disease research, where precise receptor-ligand interaction studies are paramount.

Application

• Immunology Research: As a standard agonist for the Formyl Peptide Receptor (FPR/FPRL1) to study neutrophil activation, chemotaxis, and superoxide production.
• Inflammation Models: Used to induce and investigate acute inflammatory responses in various in vitro and in vivo experimental systems.
• Drug Discovery & Screening: Serves as a reference compound and positive control in high-throughput screens for identifying novel FPR antagonists or modulators for anti-inflammatory therapeutics.
• Signal Transduction Studies: Employed to elucidate downstream signaling cascades (e.g., calcium mobilization, MAPK activation) triggered by G-protein coupled receptor (GPCR) engagement.
• Cell Biology Assays: Utilized in Boyden chamber or under-agarose assays to quantitatively measure directed cell migration (chemotaxis) of phagocytes.
• Biochemical Reagent: Acts as a key ligand in receptor binding assays and for the characterization of FPR expression and affinity.

Basic Information

Product Name	n-Formyl-Met-Leu-Phe
CAS No.	5979-11-3
Molecular Formula	C21H31N3O5S
Molecular Weight	437.55 g/mol
Synonyms	N-Formylmethionyl-leucyl-phenylalanine; fMLP; fMet-Leu-Phe; Formyl-Met-Leu-Phe; Chemotactic Peptide fMLP; N-Formyl-Met-Leu-Phe-OH; Formylmethionylleucylphenylalanine; For-Met-Leu-Phe
EINECS	Contact for details

Quality Control

Our n-Formyl-Met-Leu-Phe (CAS 5979-11-3) is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, MS for identity confirmation, and chiral purity assessment. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 80.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.