Description

Biocytin is a high-purity biochemical reagent, a biotinylated lysine derivative that serves as a critical intermediate and research tool. Its primary value lies in its ability to act as a substrate for biotinidase and as a versatile precursor for introducing biotin tags into biological systems, enabling sensitive detection and isolation protocols. This compound is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science research sectors, particularly for applications involving enzyme studies, histochemistry, and molecular probe development.

Application

• Enzyme Substrate for Biotinidase: Used in biochemical assays to study biotinidase enzyme activity and diagnose related metabolic disorders.
• Neuroscience Research: Employed as a tracer in neuroanatomical studies, where it is taken up by neurons and transported, allowing for neural pathway mapping.
• Molecular Biology Probe Synthesis: Serves as a key precursor for synthesizing biotin-labeled nucleotides, peptides, and other probes for blotting, hybridization, and affinity purification.
• Diagnostic Kit Manufacturing: Integrated as a critical component in the production of ELISA and other immunoassay kits that utilize streptavidin-biotin technology for signal amplification.
• Bioconjugation and Labeling: Facilitates the biotinylation of antibodies, proteins, and other molecules for detection and pull-down assays in proteomics and cell biology.
• Pharmaceutical Intermediate: Utilized in the research and development stages of novel therapeutic agents that target biotin-dependent pathways.

Basic Information

Product Name	Biocytin
CAS No.	576-19-2
Molecular Formula	C16H28N4O4S
Molecular Weight	372.48 g/mol
Synonyms	Nε-Biotinyl-L-lysine; Biotinyl-L-lysine; L-Lysine, N6-biotinyl-; (+)-Biocytin; D-Biocytin; Biotin-L-lysine; Vitamin H-lysine; Biotinyllysine; NSC 114044
EINECS	209-406-8

Quality Control

Our Biocytin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines and can provide material to meet specific customer requirements for research or cGMP-grade applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Optical Rotation	[α]20/D: +20° to +24° (c=1 in 0.1N NaOH)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.