Description

2-[15N]Acetamido-2-Deoxy-D-[1-13C]Glucose is a high-purity, dual-labeled isotopologue of N-acetylglucosamine, a fundamental building block in glycobiology and metabolic research. This compound is specifically engineered for advanced tracer studies and NMR spectroscopy, enabling precise tracking of metabolic pathways and structural elucidation in complex biological systems. It is an essential reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require stable isotope-labeled compounds for drug development, biomarker discovery, and metabolic flux analysis.

Application

• Metabolic Pathway Tracing: Used as a 13C and 15N dual-labeled tracer to study glycosylation processes, hexosamine biosynthesis, and carbohydrate metabolism in cell cultures and animal models.
• NMR Spectroscopy Standard: Serves as an internal standard or reference compound for Nuclear Magnetic Resonance (NMR) analysis due to its distinct isotopic signature, aiding in the structural characterization of glycans and glycoconjugates.
• Pharmaceutical R&D: Employed in the research and development of glycosidase inhibitors, anti-cancer agents, and therapies targeting metabolic disorders where glucosamine metabolism is implicated.
• Biomarker Research: Facilitates the development of quantitative mass spectrometry assays for detecting and measuring N-acetylglucosamine and related metabolites in clinical samples.
• Glycoprotein & Proteomics Studies: Essential for investigating post-translational modifications, specifically O-GlcNAcylation, which plays a critical role in cell signaling and regulation.
• Enzyme Mechanism Studies: Utilized to elucidate the catalytic mechanisms of glycosyltransferases, glycosidases, and other enzymes involved in carbohydrate processing.

Basic Information

Product Name	2-[15N]Acetamido-2-Deoxy-D-[1-13C]Glucose
CAS No.	478529-40-7
Molecular Formula	C7H1315N13CO5
Molecular Weight	~221.18 g/mol (for 13C1, 15N1 isotopologue)
Synonyms	N-Acetyl-D-[1-13C]glucosamine-15N; 2-Acetamido-2-deoxy-D-[1-13C]glucose-15N; [1-13C,15N]-N-Acetylglucosamine; GlcNAc-1-13C,15N; 2-(15N)Acetamido-2-deoxy-D-(1-13C)glucose; Stable Isotope Labeled N-Acetylglucosamine; NAG-13C1,15N1
EINECS	Contact for details

Quality Control

Every batch of 2-[15N]Acetamido-2-Deoxy-D-[1-13C]Glucose is manufactured and analyzed under strict quality systems to ensure exceptional isotopic and chemical purity. Our rigorous quality testing includes advanced spectroscopic and chromatographic methods to verify identity, isotopic enrichment, and purity, ensuring compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing isotopic purity (atom % 13C and 15N), chemical purity, and analytical results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Purity (13C)	≥ 99 atom % 13C
Isotopic Purity (15N)	≥ 98 atom % 15N
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.