Description

Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-Nh2 is a synthetic, fluorescently labeled peptide substrate designed for high-sensitivity biochemical research. This compound matters for its specific role in studying and quantifying the activity of key proteolytic enzymes, such as furin and related proprotein convertases, which are critical in numerous physiological and pathological processes. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic developers focused on enzyme kinetics, inhibitor screening, and cellular signaling pathway analysis.

Application

• Enzyme Activity Assays: Primary substrate for the fluorometric determination of furin and PC (proprotein convertase) enzyme activity in vitro.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel inhibitors of furin-like enzymes for therapeutic development.
• Biochemical Research: A critical tool for investigating the proteolytic processing of viral glycoproteins (e.g., HIV-1 gp160, SARS-CoV-2 Spike) and mammalian proproteins.
• Diagnostic Development: Serves as a core component in developing sensitive diagnostic kits for diseases where furin activity is dysregulated.
• Cell Signaling Studies: Enables research into pathways involving growth factors, hormones, and receptors that require proprotein convertase-mediated activation.
• Quality Control in Biologics: Can be utilized in QC labs to monitor the presence and activity of specific host cell proteases during biopharmaceutical production.

Basic Information

Item	Detail
Product Name	Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-Nh2
CAS No.	438625-61-7
Molecular Formula	C86H130N30O26
Molecular Weight	1919.1 g/mol (theoretical)
Synonyms	Furin Substrate, Mca-RTKR-2,4-Dnp; 7-Methoxycoumarin-4-acetyl-Arg-Thr-Lys-Arg-2,4-Dinitrophenyl; Fluorogenic Furin Substrate; Pyrfurin; (7-Methoxycoumarin-4-yl)acetyl-Arg-Thr-Lys-Arg-2,4-dinitrophenyl; PC Substrate; Proprotein Convertase Substrate
EINECS	Contact for details

Quality Control

Our Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-Nh2 is manufactured under strict quality systems suitable for research use. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistent performance and reliability in sensitive assay systems. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70% (by weight)
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.