Description

H-Glu(Edans)-Val-Tyr-Leu-Lys-Ala-Ser-Gln-Phe-Pro-Ala-Gly-Ile-Lys(Dabcyl)-Gly-Oh is a specialized, high-purity fluorescent peptide substrate designed for sensitive protease activity assays. This compound is critical for researchers and developers in life sciences and pharmaceutical discovery, enabling precise, real-time monitoring of enzymatic cleavage events. It is primarily utilized by biotech, pharmaceutical, and academic research institutions focused on protease characterization, inhibitor screening, and diagnostic assay development.

Application

• Protease Activity Assays: As a FRET (Fluorescence Resonance Energy Transfer)-based substrate for continuous, real-time kinetic measurements of protease activity.
• Drug Discovery & Screening: High-throughput screening (HTS) of potential protease inhibitors for therapeutic development in areas like oncology and virology.
• Enzyme Characterization: Determining kinetic parameters (Km, kcat) and specificity of proteases such as caspases, thrombin, or other serine/cysteine proteases.
• Diagnostic Development: A key component in the development of sensitive in vitro diagnostic (IVD) tests for disease biomarkers.
• Biochemical Research: Fundamental research into protease signaling pathways, apoptosis studies, and protein biochemistry.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	H-Glu(Edans)-Val-Tyr-Leu-Lys-Ala-Ser-Gln-Phe-Pro-Ala-Gly-Ile-Lys(Dabcyl)-Gly-Oh
CAS No.	400746-50-1
Molecular Formula	C99H136N22O28
Molecular Weight	~2009.3 g/mol
Synonyms	EDANS-DABCYL FRET Peptide Substrate; Caspase-3 Substrate II, Fluorogenic; Ac-DEVD-AMC Analog FRET Substrate; Glu(EDANS)-Val-Tyr-Leu-Lys(DABCYL)-Gly; FRET-based Protease Substrate; Fluorogenic Peptide Substrate for Caspase-3; Dabcyl-Glu-Val-Tyr-Leu-Lys(Edans)-Gly; Protease Fluorescent Substrate 400746-50-1
EINECS	Contact for details

Quality Control

Every batch of this high-purity peptide substrate is manufactured and tested under strict quality management systems. We guarantee consistent performance and lot-to-lot reproducibility for critical research applications. Full analytical characterization is performed, including HPLC for purity and MS for identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, performance data (e.g., fluorescence quenching efficiency), and analytical methods is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into single-use quantities to minimize freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; Limited solubility in aqueous buffers
Fluorescence Properties	Ex/Em: ~340 nm / 490 nm (upon cleavage)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.