Description

H-Pro-His-Glu-Oh is a high-purity tripeptide derivative, specifically the C-terminal carboxylic acid form of the sequence Proline-Histidine-Glutamic Acid. This compound serves as a critical building block and reference standard in advanced biochemical research and pharmaceutical development. It is primarily utilized by researchers and manufacturers in the fields of peptide synthesis, medicinal chemistry, and proteomics for studying enzyme-substrate interactions, designing novel therapeutics, and developing diagnostic assays.

Application

• Pharmaceutical Intermediates: As a key synthon in the solid-phase and solution-phase synthesis of more complex bioactive peptides and peptidomimetics.
• Biochemical Research: Used as a reference standard or substrate analog in enzymatic studies, particularly those involving metalloproteases or histidine-dependent catalytic mechanisms.
• Medicinal Chemistry: Employed in structure-activity relationship (SAR) studies to optimize peptide-based drug candidates for enhanced stability, bioavailability, and target affinity.
• Proteomics & Diagnostics: Potential use in the development of specific assays, affinity ligands, or calibration standards for protein analysis.
• Academic & Institutional Research: A valuable tool for universities and research institutes investigating peptide structure, function, and metabolism.

Basic Information

Product Name	H-Pro-His-Glu-Oh
CAS No.	47555-31-7
Molecular Formula	C17H24N5O7
Molecular Weight	410.40 g/mol
Synonyms	L-Prolyl-L-histidyl-L-glutamic acid; Pro-His-Glu; Proline-Histidine-Glutamic Acid; PHG; H-Pro-His-Glu-OH; L-Pro-L-His-L-Glu; N-(N-(N-L-Prolyl-L-histidyl))-L-glutamic acid; 2-[[(2S)-1-[(2S)-2-[[(2S)-1-hydroxy-5-oxopyrrolidine-2-carbonyl]amino]-3-(1H-imidazol-4-yl)propanoyl]pyrrolidin-2-yl]formamido]pentanedioic acid
EINECS	Contact for details

Quality Control

Every batch of H-Pro-His-Glu-Oh is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality assurance protocols are designed to meet the stringent requirements of research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity (typically by HPLC), chiral purity, and other critical parameters is provided with each shipment to guarantee traceability and reliability for your projects.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Chiral Purity (HPLC)	≥ 99.0% (all L-isomers)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.