Description

Ac-Asp-Pna CAS NO 41149-01-3 is a specialized peptide derivative, specifically N-Acetyl-L-aspartyl-p-nitroanilide, designed for high-precision biochemical research and process development. This compound serves as a critical chromogenic substrate for the sensitive detection and kinetic study of specific proteolytic enzymes, including aspartyl proteases. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors requiring reliable, high-purity substrates for enzymatic assays.

Application

• Enzyme Substrate for Protease Assays: Primary use as a chromogenic substrate for the spectrophotometric determination of aspartyl protease activity.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibition profiling to understand protease function and mechanism.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) campaigns to identify and optimize novel protease inhibitors as potential drug candidates.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical and research purposes targeting specific proteases.
• Quality Control in Bioprocessing: Monitoring protease activity during fermentation, cell culture, and downstream purification processes in biomanufacturing.

Basic Information

Product Name	Ac-Asp-Pna
CAS No.	41149-01-3
Molecular Formula	C₁₄H₁₈N₄O₆
Molecular Weight	338.32 g/mol
Synonyms	N-Acetyl-L-aspartyl-p-nitroanilide; Ac-Asp-pNA; N-Ac-Asp-pNA; N-Acetyl-L-aspartic acid p-nitroanilide; L-Aspartic acid, N-acetyl-, 4-nitroanilide; Acetyl-Asp-p-nitroanilide; Protease substrate Ac-Asp-pNA; Chromogenic substrate for aspartyl proteases
EINECS	Contact for details

Quality Control

Our Ac-Asp-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent in-house specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.