Description

Phosphohistidine CAS NO 4936-08-7 is a crucial phosphorylated derivative of the amino acid histidine, playing a fundamental role in cellular signal transduction and enzyme regulation. This compound is essential for researchers studying phosphohistidine phosphatases and kinases, as it serves as a key substrate and reference standard. It is primarily needed by biochemical and pharmaceutical R&D laboratories focused on post-translational modifications, metabolic pathway analysis, and the development of targeted therapeutics.

Application

• Biochemical Research: Used as a critical substrate and reference standard for studying phosphohistidine phosphatases (PHPs) and kinases involved in signal transduction.
• Enzyme Mechanism Studies: Essential for investigating the role of histidine phosphorylation in enzyme catalysis and regulation across various metabolic pathways.
• Pharmaceutical Development: Serves as a key intermediate and tool compound in drug discovery programs targeting phosphohistidine-related pathways, such as in oncology and metabolic disorders.
• Diagnostic Reagent Production: Employed in the development and calibration of diagnostic assays and kits designed to detect histidine phosphorylation events.
• Cell Signaling & Proteomics: Utilized in proteomic studies to identify and characterize proteins that undergo histidine phosphorylation, a vital but less-studied post-translational modification.
• Academic Research: Fundamental for university and institutional research into two-component signaling systems in bacteria and other novel phospho-relay mechanisms.

Basic Information

Product Name	Phosphohistidine
CAS No.	4936-08-7
Molecular Formula	C6H10N3O5P
Molecular Weight	235.14 g/mol
Synonyms	1-Phosphohistidine; Histidine 1-phosphate; L-Histidine, 1-(dihydrogen phosphate); 1-Phospho-L-histidine; Phospho-His; His-P; (2S)-2-Amino-3-(1-phosphono-1H-imidazol-4-yl)propanoic acid
EINECS	225-574-5

Quality Control

Our Phosphohistidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed under desiccant and allowed to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on anhydrous basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.