Description

Phenylthiohydantoin-Dl-Alanine is a specialized derivative of alanine, a fundamental amino acid, modified with a phenylthiohydantoin group. This modification is critical for its primary role as a key intermediate and reference standard in advanced peptide sequencing and amino acid analysis. It is essential for research and quality control laboratories in the pharmaceutical and biotechnology sectors that require precise analytical tools. The compound's specific structure makes it valuable for developing and validating chromatographic and spectroscopic methods.

Application

• Peptide Sequencing Reagent: Used as a critical intermediate in the Edman degradation process for determining the amino acid sequence of peptides and proteins.
• Analytical Reference Standard: Serves as a high-purity standard for calibrating equipment and validating methods in High-Performance Liquid Chromatography (HPLC) and mass spectrometry.
• Biochemical Research: Employed in studies investigating amino acid metabolism, protein structure-function relationships, and enzyme mechanisms.
• Pharmaceutical Development: Utilized in the synthesis and quality control of peptide-based active pharmaceutical ingredients (APIs) and therapeutic agents.
• Proteomics and Genomics: Applied in core facilities for protein identification and characterization workflows.
• Diagnostic Kit Manufacturing: Acts as a component or calibrant in diagnostic assays designed to detect specific proteins or metabolic markers.

Basic Information

Product Name	Phenylthiohydantoin-Dl-Alanine
CAS No.	4333-19-1
Molecular Formula	C₁₂H₁₂N₂O₂S
Molecular Weight	248.30 g/mol
Synonyms	PTH-DL-Alanine; DL-Alanine, N-(phenylthiohydantoinyl)-; 3-(2-Phenyl-2-thioxo-1-imidazolidinyl)-2-aminopropanoic acid; 2-Amino-N-(2-phenyl-4-thioxo-1-imidazolidinyl)propionamide; Edman Degradation derivative of DL-Alanine; Alanine PTH derivative; Phenylthiohydantoin alanine; Alanine, phenylthiohydantoin-
EINECS	Contact for details

Quality Control

Our Phenylthiohydantoin-Dl-Alanine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and analytical applications. Certificates of Analysis (COA) with detailed batch-specific results are provided to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed in a dry environment to minimize exposure to moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.