Description

Dihydrofolic Acid CAS NO 4033-27-6 is a vital biochemical intermediate in the folate metabolic pathway. This compound serves as a key precursor in the synthesis of tetrahydrofolate, an essential cofactor in one-carbon transfer reactions critical for nucleotide synthesis and amino acid metabolism. It is primarily utilized by research institutions and manufacturers in the pharmaceutical, biochemical, and life science sectors for advanced research and development.

Application

• Biochemical Research: Critical intermediate for studying folate metabolism, enzyme kinetics, and one-carbon transfer pathways in academic and industrial laboratories.
• Pharmaceutical Development: Used as a reference standard and a key starting material or intermediate in the synthesis of novel antifolate drugs and therapeutic agents.
• Enzymology Studies: Essential substrate for investigating the activity and inhibition of dihydrofolate reductase (DHFR), a major target in cancer and antimicrobial chemotherapy.
• Nutritional Science Research: Employed in metabolic studies to understand folate bioavailability, biosynthesis, and its role in cellular processes.
• Diagnostic Reagent Manufacturing: Serves as a component in the formulation of specialized diagnostic kits and assays for clinical and research purposes.

Basic Information

Product Name	Dihydrofolic Acid
CAS No.	4033-27-6
Molecular Formula	C19H21N7O6
Molecular Weight	443.41 g/mol
Synonyms	7,8-Dihydrofolic Acid; 7,8-Dihydropteroylglutamic Acid; DHFA; NSC 52907; Dihydrofolate; 2-[[4-[[(2-Amino-7,8-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]amino]pentanedioic acid
EINECS	Contact for details

Quality Control

Our Dihydrofolic Acid is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development applications. A detailed Certificate of Analysis (COA) is provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (e.g., nitrogen or argon) at a controlled room temperature (15-25°C) or as specified on the label or COA. This product is light-sensitive and easily oxidized; prolonged exposure to air, light, or elevated temperatures should be avoided to maintain stability and purity.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Related Substances (HPLC)	Total impurities ≤ 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.