Description

Abz-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-p-Nitro-Phe-Nh2 is a synthetic, custom-designed peptide sequence featuring a fluorescent aminobenzoic acid (Abz) group and a p-nitro-phenylalanine residue. This compound is a critical research tool for studying protease activity and enzyme kinetics, particularly in high-throughput screening and biochemical assay development. It is primarily utilized by pharmaceutical R&D departments, academic research institutions, and biotechnology companies focused on drug discovery and proteomics.

Application

• Protease Substrate & Enzyme Assays: Serves as a specialized fluorogenic or chromogenic substrate for the study of specific proteolytic enzymes, including serine and cysteine proteases.
• Drug Discovery & High-Throughput Screening (HTS): Used in the development and validation of assays to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: A key reagent for investigating enzyme mechanisms, specificity, and kinetic parameters (Km, Vmax, kcat) in controlled laboratory studies.
• Diagnostic Kit Development: Employed in the formulation of research-grade diagnostic kits for detecting and quantifying specific protease activities in biological samples.
• Peptide Chemistry & Custom Synthesis: Acts as a reference standard or an intermediate in the synthesis of more complex peptide-based molecules for advanced research.

Basic Information

Product Name	Abz-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-p-Nitro-Phe-Nh2
CAS No.	396096-53-0
Molecular Formula	C58H78N12O19
Molecular Weight	1239.3 g/mol
Synonyms	Abz-ETLFQGPY-pNO2-F-NH2; 2-Aminobenzoic acid-L-glutamyl-L-threonyl-L-leucyl-L-phenylalanyl-L-glutaminyl-glycyl-L-prolyl-L-valyl-4-nitro-L-phenylalaninamide; Fluorogenic Peptide Substrate Abz-ETLFQGPY-pNO2-F-NH2; Custom Peptide 396096-53-0; Protease Substrate Peptide; p-Nitro-Phe containing peptide; Abz labeled peptide
EINECS	Contact for details

Quality Control

Every batch of Abz-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-p-Nitro-Phe-Nh2 is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality assurance protocol is designed to meet the exacting standards of research and development. Certificates of Analysis (COA) detailing purity (by HPLC), molecular weight confirmation (MS), and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Single Impurity (HPLC)	≤ 1.0%
Amino Acid Analysis	Within ± 10% of theoretical
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.