Description

H-Gly-Gly-Arg-Anilide is a specialized peptide derivative, specifically an anilide of the tripeptide glycyl-glycyl-arginine, serving as a key biochemical tool and synthetic intermediate. Its primary value lies in its role as a chromogenic substrate for the sensitive detection and kinetic study of serine proteases, particularly trypsin and trypsin-like enzymes. This compound is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science research sectors, where precise enzyme activity measurement is critical for drug discovery, quality control, and biochemical assay development.

Application

• Chromogenic Substrate for Protease Assays: Primarily used as a specific substrate for the spectrophotometric determination of trypsin, thrombin, and other serine protease activities.
• Biochemical Research & Drug Discovery: Employed in high-throughput screening (HTS) platforms to identify and characterize protease inhibitors as potential therapeutic agents.
• Diagnostic Kit Manufacturing: Integrated as a critical reagent in clinical diagnostic kits for measuring pancreatic function and other protease-related physiological conditions.
• Enzyme Kinetics Studies: Utilized to study enzyme mechanism, specificity, and kinetic parameters (Km, Vmax) in academic and industrial research settings.
• Quality Control in Bioprocessing: Applied to monitor and validate the activity of protease enzymes used in biotechnological manufacturing processes.

Basic Information

Product Name	H-Gly-Gly-Arg-Anilide
CAS No.	390394-20-4
Molecular Formula	C17H26N8O4
Molecular Weight	406.44 g/mol
Synonyms	Gly-Gly-Arg-p-nitroanilide; GGR-pNA; H-Glycyl-glycyl-L-arginine 4-nitroanilide; Nα-Benzoyl-Gly-Gly-Arg-p-nitroanilide (common derivative); Bz-Gly-Gly-Arg-pNA; Trypsin chromogenic substrate; Protease substrate 390394-20-4; L-Arginine, N2-[N-(glycylglycyl)]-, 4-nitroanilide
EINECS	Contact for details

Quality Control

Our H-Gly-Gly-Arg-Anilide is produced and tested under a strict quality management system to ensure batch-to-batch consistency and high performance in sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant GMP guidelines for laboratory chemicals to guarantee suitability for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Functional Activity (Trypsin Assay)	≥ 90% of reference standard
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.