Description

L-Serine-n-Fmoc, o-tert-Butyl Ether (15N) CAS NO 387867-79-0 is a stable isotope-labeled derivative of the amino acid serine, featuring both Fmoc (9-fluorenylmethoxycarbonyl) protection on the amine and a tert-butyl ether group on the side-chain hydroxyl. This compound is specifically designed for advanced peptide synthesis and proteomics research, where the 15N label enables precise tracking and quantification using mass spectrometry. It is an essential building block for pharmaceutical R&D, particularly in the development of labeled peptides, metabolic studies, and as an internal standard for analytical methods.

Application

• Isotope-Labeled Peptide Synthesis: Critical building block for the solid-phase and solution-phase synthesis of 15N-labeled peptides for mass spectrometry-based proteomics and quantitative analysis.
• Metabolic Pathway Research: Used as a tracer to study serine metabolism, biosynthesis, and utilization in biological systems using NMR or LC-MS techniques.
• Pharmaceutical Reference Standards: Serves as a high-purity certified reference material (CRM) or internal standard for the accurate quantification of peptides and related compounds in drug development.
• Structural Biology & Biophysics: Enables the site-specific incorporation of a 15N label into proteins for nuclear magnetic resonance (NMR) spectroscopy studies of protein structure and dynamics.
• Proteomics & Biomarker Discovery: Facilitates stable isotope labeling by amino acids in cell culture (SILAC) and other quantitative proteomic strategies to compare protein expression levels.
• Chemical Biology Probes: Used in the synthesis of activity-based probes or affinity tags to study enzyme function and protein-protein interactions.

Basic Information

Product Name	L-Serine-n-Fmoc, o-tert-Butyl Ether (15N)
CAS No.	387867-79-0
Molecular Formula	C22H2515NO5
Molecular Weight	~391.44 g/mol (for 15N isotopologue)
Synonyms	Fmoc-Ser(OtBu)-OH (15N); N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-O-(tert-butyl)-L-serine-15N; Fmoc-L-serine(O-t-butyl)-15N; (2S)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl-15N]amino]-3-[(2-methylpropan-2-yl)oxy]propanoic acid; Fmoc-Ser(tBu)-OH-15N; Nα-Fmoc-O-tert-butyl-L-serine-15N; Fmoc-Ser(OtBu)-15N-OH; Fmoc-L-Ser(OtBu)-15N
EINECS	Contact for details

Quality Control

Our L-Serine-n-Fmoc, o-tert-Butyl Ether (15N) is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry to confirm isotopic enrichment, chemical identity, and purity. A comprehensive Certificate of Analysis (COA) detailing assay, isotopic purity, chiral purity, and impurity profile is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storing under an inert atmosphere in a desiccator to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Isotopic Purity (15N)	≥98 atom % 15N
Chiral Purity (HPLC)	≥99.0% (L-isomer)
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.