Description

Ac-Ile-Glu-Thr-Asp-Amc is a specialized fluorogenic peptide substrate designed for high-sensitivity protease activity assays. This compound is critical for researchers and developers requiring precise, quantitative measurement of enzymatic activity in complex biological matrices. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic kit development for studying caspase and related protease functions.

Application

• Protease Activity Assays: As a fluorogenic substrate for monitoring the activity of caspases and other aspartate-specific proteases in cell lysates and purified enzyme preparations.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Apoptosis Research: A key tool for quantifying caspase activation during programmed cell death studies in cancer research and neurobiology.
• Biochemical Research Reagent: Employed in enzyme kinetics studies to determine kinetic parameters (Km, Vmax) of specific proteases.
• Diagnostic Development: Serves as a core component in the development of clinical diagnostic assays for conditions involving dysregulated protease activity.

Basic Information

Product Name	Ac-Ile-Glu-Thr-Asp-Amc
CAS No.	348079-17-4
Molecular Formula	C31H42N6O13
Molecular Weight	706.70 g/mol
Synonyms	Ac-IETD-Amc; Ac-Ile-Glu-Thr-Asp-7-Amino-4-methylcoumarin; Acetyl-Isoleucyl-Glutamyl-Threonyl-Aspartyl-7-Amino-4-methylcoumarin; Caspase-8 Substrate, Fluorogenic; IETD-AFC (related fluorophore); Ac-IETD-AMC; ICE-Like Protease Substrate; Fluorogenic Caspase-8 Substrate IETD-Amc
EINECS	Contact for details

Quality Control

Our Ac-Ile-Glu-Thr-Asp-Amc is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and fluorometric validation of functional activity. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity (Fluorometric Assay)	Conforms to reference standard
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.