Description

n-Succinimidyl 4-(2-Pyridyldithio)Pentanoate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a sulfhydryl-reactive pyridyldithiol group. This compound is critical for creating stable, cleavable conjugates between proteins, peptides, and other biomolecules, enabling advanced bioconjugation strategies. It is an essential tool for researchers and manufacturers in biopharmaceutical development, antibody-drug conjugate (ADC) production, and diagnostic assay development who require precise and reversible linkage chemistry.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for constructing cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein-Protein/Peptide Conjugation: For crosslinking amine-containing molecules (e.g., lysine residues) with thiol-containing partners to study interactions or create novel protein constructs.
• Surface Immobilization & Bioconjugation: Functionalizing solid supports (e.g., sensor chips, magnetic beads) with biomolecules for diagnostics, biosensors, and affinity purification.
• Fluorescent & Enzymatic Labeling: Attaching reporter molecules (fluorophores, enzymes) to antibodies, proteins, or oligonucleotides for imaging and detection applications.
• Polymer & Biomaterial Modification: Introducing reactive handles onto polymers or hydrogels to enable subsequent coupling with bioactive molecules.
• Stable & Cleavable Bond Formation: Creating disulfide bonds that are stable in circulation but can be selectively cleaved in reducing intracellular environments.

Basic Information

Product Name	n-Succinimidyl 4-(2-Pyridyldithio)Pentanoate
CAS No.	341498-08-6
Molecular Formula	C14H15N2O4S2
Molecular Weight	339.41 g/mol
Synonyms	SPDP; N-Succinimidyl 3-(2-pyridyldithio)propionate (common older name); SPP; 4-(2-Pyridinyldithio)pentanoic Acid N-Hydroxysuccinimide Ester; 3-(2-Pyridyldithio)propionic Acid N-Succinimidyl Ester; N-Succinimidyl 4-(2-Pyridyldithio)butyrate (related analog); LC-SPDP; Succinimidyl Pyridyl Dithio Propionate
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 4-(2-Pyridyldithio)Pentanoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive bioconjugation applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and functional activity validation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.