Description

[Glu27]-Pkc (19-36) CAS NO 309247-49-2 is a synthetic peptide fragment derived from the Protein Kinase C (PKC) enzyme, specifically corresponding to residues 19-36 with a glutamate substitution at position 27. This compound is a critical research tool for studying PKC signaling pathways, which are central to numerous cellular processes including proliferation, differentiation, and apoptosis. It is primarily utilized by researchers and scientists in the fields of biochemistry, molecular biology, and pharmaceutical development for enzyme inhibition studies, substrate specificity assays, and the investigation of intracellular signal transduction mechanisms.

Application

• Biochemical Research: Used as a specific substrate or inhibitor in enzymatic assays to study Protein Kinase C (PKC) activity and kinetics.
• Signal Transduction Studies: Essential for investigating the role of PKC in cellular signaling pathways related to cancer, cardiovascular diseases, and neurological disorders.
• Drug Discovery & Development: Serves as a key reagent in high-throughput screening (HTS) campaigns to identify and validate novel PKC-targeted therapeutic compounds.
• Academic Research: Employed in university and institutional labs for fundamental research on protein-protein interactions and post-translational modifications.
• Diagnostic Development: Can be used in the development of diagnostic assays to measure PKC levels or activity in biological samples.

Basic Information

Product Name	[Glu27]-Pkc (19-36)
CAS No.	309247-49-2
Molecular Formula	C89H144N26O27S
Molecular Weight	~2009.3 g/mol
Synonyms	PKC (19-36), [Glu27] analog; PKCα (19-36), Glu27-substituted; Protein Kinase C Fragment (19-36); PKC Inhibitory Peptide; RFARKGSLRQKNVHEVKN; H-Arg-Phe-Ala-Arg-Lys-Gly-Ser-Leu-Arg-Gln-Lys-Asn-Val-His-Glu-Val-Lys-Asn-OH (Glu27); PKC Pseudosubstrate Peptide; PKC(19-31) extended analog
EINECS	Contact for details

Quality Control

Our [Glu27]-Pkc (19-36) is manufactured under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including reversed-phase HPLC for purity assessment and mass spectrometry (MS) for identity confirmation. Certificates of Analysis (COA) detailing purity, amino acid analysis, and peptide content are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70%
Amino Acid Analysis	± 10% of theoretical
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.