Description

n-Fmoc-γ-Carboxyglutamic Acid γ,γ-Di-T-Butyl Ester CAS NO 308357-14-4 is a sophisticated, orthogonally protected derivative of glutamic acid, specifically engineered for advanced peptide synthesis. This compound matters because it provides critical control over the reactive γ-carboxyl group, enabling the precise incorporation of γ-carboxyglutamic acid (Gla) residues, which are essential for the biological activity of numerous proteins. It is primarily needed by research institutions and pharmaceutical companies engaged in the development of peptide-based therapeutics, vitamin K-dependent coagulation factors, and bone matrix proteins.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key building block for the controlled introduction of γ-carboxyglutamic acid (Gla) residues into peptide chains, crucial for mimicking natural post-translational modifications.
• Pharmaceutical Research & Development: Used in the synthesis of peptide analogs targeting blood coagulation (e.g., Factor IX, Protein C), bone metabolism (e.g., osteocalcin), and other Gla-dependent biological pathways.
• Bioconjugation & Probe Development: Serves as a protected intermediate for creating labeled or functionalized peptides for diagnostic assays and biochemical studies.
• Process Chemistry & Scale-Up: Employed in the development and manufacturing of active pharmaceutical ingredients (APIs) that require Gla domains for therapeutic efficacy.
• Academic Research: Fundamental tool for studying the structure-activity relationships of vitamin K-dependent proteins and calcium-binding motifs in peptides.

Basic Information

Product Name	n-Fmoc-γ-Carboxyglutamic Acid γ,γ-Di-T-Butyl Ester
CAS No.	308357-14-4
Molecular Formula	C29H35NO8
Molecular Weight	525.60 g/mol
Synonyms	Fmoc-Gla(OtBu)2-OH; N-α-Fmoc-N-γ,γ-di-tert-butyl glutamic acid; Fmoc-γ-carboxyglutamic acid γ,γ-di-tert-butyl ester; N-(9-Fluorenylmethoxycarbonyl)-γ-carboxyglutamic acid γ,γ-di-tert-butyl ester; Fmoc-Gla(OtBu)2; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]pentanedioic acid 5,5-di-tert-butyl ester; Protected Fmoc-Glutamic Acid Derivative; Peptide Synthesis Building Block
EINECS	Contact for details

Quality Control

Our n-Fmoc-γ-Carboxyglutamic Acid γ,γ-Di-T-Butyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for sensitive peptide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and traceability for your R&D and cGMP processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.