Description

H-His(1-Trt)-Otbu is a protected derivative of the amino acid L-Histidine, specifically designed for use in solid-phase peptide synthesis (SPPS). This compound is critical for researchers and manufacturers requiring precise control over the imidazole side chain of histidine during complex peptide assembly. It is primarily utilized by pharmaceutical R&D teams, biotech companies, and fine chemical suppliers engaged in the development of therapeutic peptides, diagnostic agents, and biochemical tools.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a key building block for incorporating histidine residues with orthogonal side-chain protection, preventing unwanted side reactions.
• Therapeutic Peptide Production: Used in the research-scale and commercial synthesis of peptide-based drugs, including hormones, antimicrobial peptides, and enzyme inhibitors.
• Combinatorial Chemistry Libraries: Serves as a valuable component for generating diverse peptide libraries for high-throughput screening in drug discovery.
• Bioconjugation and Labeling: The protected functional groups allow for selective deprotection and subsequent conjugation with reporter molecules or other biomolecules.
• Academic and Industrial Research: A fundamental reagent for studying protein structure-function relationships, enzyme mechanisms, and peptide mimetics.

Basic Information

Product Name	H-His(1-Trt)-Otbu
CAS No.	304698-96-2
Molecular Formula	C33H35N3O2
Molecular Weight	505.65 g/mol
Synonyms	N(α)-tert-Butoxycarbonyl-N(π)-trityl-L-histidine; Boc-His(Trt)-OH; (2S)-2-[(tert-Butoxy)carbonylamino]-3-[1-(triphenylmethyl)-1H-imidazol-4-yl]propanoic acid; Boc-L-histidine(N-π-trityl); tert-Butyloxycarbonyl-N(π)-trityl-L-histidine; Boc-His(Trt)-OH; L-Histidine, N2-[(1,1-dimethylethoxy)carbonyl]-N(pi)-triphenylmethyl-
EINECS	Contact for details

Quality Control

Our H-His(1-Trt)-Otbu is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis for enantiomeric excess, and NMR for structural confirmation. A Certificate of Analysis (COA) detailing identity, purity, and specific impurity profiles is provided with every shipment to ensure compliance with research and cGMP guidelines for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This material is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere (argon or nitrogen) after each use to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Specific Rotation	-5.0° to -7.0° (c=1 in DMF)
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.