Description

Histone, Calf Thymus is a complex mixture of basic nuclear proteins, primarily comprising the core histones (H2A, H2B, H3, H4) and linker histone (H1), isolated from calf thymus tissue. This product is a fundamental biochemical reagent essential for research in chromatin structure, epigenetics, and gene regulation. It is a critical material for scientists and industrial researchers in the fields of molecular biology, biochemistry, and pharmaceutical development, particularly for use as a substrate in enzymatic assays, nucleosome reconstitution studies, and antibody production.

Application

• Chromatin Research & Nucleosome Assembly: Used as a core component for in vitro reconstitution of nucleosomes to study chromatin structure and dynamics.
• Enzymatic Substrate: Serves as a substrate for histone-modifying enzymes such as acetyltransferases (HATs), deacetylases (HDACs), methyltransferases, and kinases in activity assays and inhibitor screening.
• Biochemical Standard: Employed as a standard or control in electrophoresis (e.g., SDS-PAGE, AU-PAGE), Western blotting, and mass spectrometry for histone protein analysis.
• Antibody Production & Validation: Used as an immunogen for generating polyclonal or monoclonal antibodies against histones, and for testing antibody specificity.
• Epigenetics Studies: Fundamental for investigating post-translational modifications (PTMs) like acetylation, methylation, and phosphorylation that regulate gene expression.
• Drug Discovery: Applied in high-throughput screening platforms to identify and characterize novel therapeutics targeting epigenetic regulators.

Basic Information

Product Name	Histone, Calf Thymus
CAS No.	39293-25-9
Molecular Formula	Mixture of proteins
Molecular Weight	Varies by histone type (approx. 11-21 kDa)
Synonyms	Calf Thymus Histones; Histone Preparation from Calf Thymus; Histone Proteins (Calf Thymus); Histone Mixture (Calf Thymus); Type II-S Histones (Calf Thymus); Total Histones (Calf Thymus); Nuclear Basic Proteins (Calf Thymus)
EINECS	Contact for details

Quality Control

Our Histone, Calf Thymus is produced and tested under stringent quality management protocols. Each lot is analyzed to ensure high purity, consistent composition, and biological activity suitable for research applications. Certificates of Analysis (COA) detailing purity, protein concentration, and electrophoretic profile are provided and available upon request, supporting compliance with rigorous laboratory standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize condensation and moisture uptake. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Electrophoresis)	Characteristic band pattern for core histones (H2A, H2B, H3, H4) and H1
Protein Content (Biuret)	≥ 95%
Solubility	Soluble in water or dilute acid (e.g., 10 mM HCl)
Purity (SDS-PAGE)	Major histone bands constitute ≥ 90% of total protein
Moisture (Karl Fischer)	≤ 5.0 %
Biological Activity	Suitable as substrate for histone-modifying enzymes

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.