Description

Dnpyr-L-Histidine is a protected derivative of the essential amino acid L-Histidine, where the imidazole nitrogen is blocked by a 2,4-dinitrophenyl (Dnpyr) group. This modification is critical for peptide synthesis, preventing unwanted side reactions and enabling selective deprotection strategies. It is an indispensable building block for researchers and manufacturers in pharmaceutical development, biochemical research, and advanced peptide production.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected intermediate for the controlled incorporation of histidine into complex peptide sequences.
• Pharmaceutical Intermediates: Used in the research and development of peptide-based therapeutics and active pharmaceutical ingredients (APIs).
• Biochemical Research: Serves as a crucial reagent for studying enzyme mechanisms, protein-protein interactions, and histidine's role in biological systems.
• Custom Peptide Manufacturing: Essential for contract research and manufacturing organizations (CROs/CMOs) producing bespoke peptides for drug discovery and diagnostics.
• Protecting Group Chemistry: A model compound for studying and developing novel protection and deprotection methodologies in organic synthesis.

Basic Information

Product Name	Dnpyr-L-Histidine
CAS No.	35899-64-0
Molecular Formula	C12H13N5O6
Molecular Weight	323.26 g/mol
Synonyms	Nim-(2,4-Dinitrophenyl)-L-histidine; DNP-L-Histidine; L-Histidine, N-(2,4-dinitrophenyl)-; (2S)-2-Amino-3-[1-(2,4-dinitrophenyl)-1H-imidazol-4-yl]propanoic acid; Dnp-His-OH; Nπ-DNP-L-histidine; Boc-His(Dnp)-OH (when used with Boc protection on the α-amino group); Fmoc-His(Dnp)-OH (when used with Fmoc protection on the α-amino group)
EINECS	Contact for details

Quality Control

Our Dnpyr-L-Histidine CAS NO 35899-64-0 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis to confirm enantiomeric integrity, and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to ensure traceability and compliance with your research or cGMP guidelines.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or light should be avoided to maintain stability and purity.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Optical Rotation	[α]D20: Specific range provided on COA
Chiral Purity (HPLC)	≥ 99.0% L-isomer
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.