Description

N2-[n-[n-[1-[[[4-(Phenylazo)Phenyl]Methoxy]Carbonyl]-L-Prolyl]-L-Phenylalanyl]Glycyl]-D-Arginine is a specialized, high-purity synthetic peptide derivative designed for advanced research and development applications. Its complex structure, featuring an azo-phenyl chromophore, makes it a valuable tool for studying enzyme-substrate interactions, particularly in protease and peptidase research. This compound is essential for pharmaceutical R&D teams, academic researchers, and fine chemical manufacturers focused on developing novel therapeutic agents, diagnostic probes, and biochemical tools. Its precise synthesis and characterization are critical for ensuring reliable and reproducible experimental outcomes.

Application

• Biochemical Research: Used as a chromogenic or fluorogenic substrate for studying the kinetics and inhibition of specific proteolytic enzymes, such as trypsin-like serine proteases.
• Pharmaceutical Development: Serves as a key intermediate or reference standard in the discovery and optimization of novel peptide-based drugs and enzyme inhibitors.
• Diagnostic Assay Development: Employed in the design of sensitive colorimetric or fluorescent assays for detecting enzyme activity in clinical or research samples.
• Proteomics & Enzymology: Acts as a selective substrate to probe the specificity and function of arginine-specific proteases within complex biological systems.
• Chemical Biology: Utilized as a molecular probe to investigate cell signaling pathways and protein-protein interactions that involve specific peptide cleavage events.
• Fine Chemical Synthesis: Functions as a sophisticated building block for the custom synthesis of more complex peptide conjugates and labeled biomolecules.

Basic Information

Product Name	N2-[n-[n-[1-[[[4-(Phenylazo)Phenyl]Methoxy]Carbonyl]-L-Prolyl]-L-Phenylalanyl]Glycyl]-D-Arginine
CAS No.	35253-75-9
Molecular Formula	C37H44N10O7
Molecular Weight	740.81 g/mol
Synonyms	D-Arg-Gly-Phe-Pro-Azo-Phe-OMe; Azo-Phenylalanine Peptide Derivative; Chromogenic Peptide Substrate 35253-75-9; p-Phenylazobenzyloxycarbonyl-Pro-Phe-Gly-D-Arg; Z-Pro-Phe-Gly-D-Arg-Azo-Phe; Azo-Phe-OMe Peptide; Protease Substrate 35253-75-9; Trypsin Chromogenic Substrate Analog
EINECS	Contact for details

Quality Control

Our N2-[n-[n-[1-[[[4-(Phenylazo)Phenyl]Methoxy]Carbonyl]-L-Prolyl]-L-Phenylalanyl]Glycyl]-D-Arginine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, MS for identity confirmation, and chiral analysis to verify stereochemical integrity. Certificates of Analysis (COA) detailing all specifications and test results are provided and can be tailored to meet specific customer or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere at lower temperatures (e.g., -20°C).

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 1.0%
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.