Description

H-His(Trt)-OH is a protected derivative of the amino acid L-Histidine, where the imidazole side chain is shielded by a trityl (Trt) protecting group. This compound is a critical building block in solid-phase peptide synthesis (SPPS), enabling the controlled assembly of complex peptides without unwanted side reactions. It is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors developing therapeutic peptides, diagnostic agents, and biochemical tools.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected amino acid for the Fmoc/t-Bu strategy, specifically for the controlled incorporation of histidine residues.
• Therapeutic Peptide Production: Used in the research and GMP manufacturing of peptide-based drugs targeting metabolic disorders, oncology, and cardiovascular diseases.
• Bioconjugation and Labeling: Serves as an intermediate for creating histidine-tagged peptides used in affinity chromatography and diagnostic assays.
• Biochemical Research: A fundamental reagent for studying peptide structure-activity relationships (SAR) and enzyme-substrate interactions.
• Antibody-Drug Conjugate (ADC) Development: Employed in the synthesis of peptide linkers for targeted drug delivery systems.
• Cosmetic Peptides: Used in the development of bioactive peptides for advanced skincare formulations.

Basic Information

Product Name	H-His(Trt)-OH
CAS No.	35146-32-8
Molecular Formula	C25H24N3O2
Molecular Weight	398.48 g/mol
Synonyms	N-α-Fmoc-N-π-trityl-L-histidine; Fmoc-His(Trt)-OH; (S)-2-(((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-3-(1-trityl-1H-imidazol-4-yl)propanoic acid; N-α-(9-Fluorenylmethyloxycarbonyl)-N-π-trityl-L-histidine; Fmoc-L-histidine(N-π-trityl)-OH; Fmoc-His(Trt); Protected L-Histidine; Fmoc-His(Trt)-OH CAS 35146-32-8
EINECS	Contact for details

Quality Control

Our H-His(Trt)-OH is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, chiral analysis for enantiomeric excess, and identity confirmation by IR and NMR spectroscopy. Certificates of Analysis (COA) with detailed chromatograms are provided to ensure compliance with your research and cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a desiccated environment to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98.5%
Specific Rotation	-10.0° to -12.0° (c=1, DMF)
Water Content (KF)	≤1.0%
Heavy Metals	≤20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.