Description

4-Azido-L-Phenylalanine (Hydrochloride) is a specialized, non-canonical amino acid derivative designed for advanced bioorthogonal chemistry applications. This compound matters because it serves as a critical building block for site-specific protein modification, enabling precise labeling and functionalization of biomolecules in complex biological systems. Researchers and developers in the fields of chemical biology, proteomics, and biopharmaceuticals need it to create novel antibody-drug conjugates (ADCs), develop diagnostic probes, and engineer proteins with tailored properties.

Application

• Bioorthogonal Labeling in Chemical Biology: Incorporation into proteins via genetic code expansion for subsequent click chemistry reactions with alkynes.
• Antibody-Drug Conjugate (ADC) Development: Enables site-specific conjugation of cytotoxic payloads to antibodies, improving therapeutic index and homogeneity.
• Proteomics and Protein Imaging: Used for fluorescent or affinity-based tagging of proteins to study localization, dynamics, and interactions in live cells.
• Material Science and Surface Engineering: Functionalization of biomaterials and surfaces with bioactive peptides or proteins.
• Enzyme and Protein Engineering: Introduction of unique chemical handles for attaching cofactors, inhibitors, or sensors to study enzyme mechanisms.
• Diagnostic Probe Synthesis: A key precursor for developing targeted imaging agents for PET, SPECT, or fluorescence-based diagnostics.

Basic Information

Product Name	4-Azido-L-Phenylalanine (Hydrochloride)
CAS No.	34670-43-4
Molecular Formula	C9H10N4O2 • HCl
Molecular Weight	242.66 g/mol (Free base: 206.20)
Synonyms	p-Azido-L-phenylalanine hydrochloride; L-4-Azidophenylalanine HCl; Fmoc-4-Azido-L-Phe-OH precursor; N3-Phe-OH • HCl; (2S)-2-Amino-3-(4-azidophenyl)propanoic acid hydrochloride; Azidophenylalanine HCl; AHA (Azido HomoAlanine) analog; Bioorthogonal amino acid
EINECS	Contact for details

Quality Control

Our 4-Azido-L-Phenylalanine (Hydrochloride) is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. While not intended as a drug substance, production follows GMP-like principles to support its use in preclinical and diagnostic development pipelines.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake. For short-term use, store in a cool, dry place (2-8°C).

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Identification (IR)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Assay (Titration)	97.0% - 103.0%
Optical Rotation	[α]D20: Contact for details
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.