Description

Ac-Phe-Gly-Pna is a specialized peptide derivative, commonly utilized as a chromogenic substrate for enzyme activity studies. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of protease and peptidase activity. It is primarily used by research institutions, diagnostic kit manufacturers, and pharmaceutical companies involved in drug discovery and enzymatic assay development.

Application

• Enzymatic Substrate: Acts as a chromogenic p-nitroaniline (Pna) substrate for assaying the activity of various proteases and peptidases.
• Biochemical Research: Used in kinetic studies and enzyme characterization to understand catalytic mechanisms and inhibition.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify potential therapeutic enzyme inhibitors.
• Diagnostic Development: Integral component in the development of colorimetric diagnostic tests for detecting specific enzyme levels.
• Protease Specificity Studies: Helps in determining the substrate specificity and optimal cleavage sites for proteolytic enzymes.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme-based products during production.

Basic Information

Item	Detail
Product Name	Ac-Phe-Gly-Pna
CAS No.	34336-99-7
Molecular Formula	C19H20N4O5
Molecular Weight	384.39 g/mol
Synonyms	Acetyl-Phenylalanyl-Glycyl-p-Nitroanilide; N-Acetyl-L-phenylalanylglycyl 4-nitroanilide; Ac-Phe-Gly-4-NA; Acetyl-Phe-Gly-pNA; Ac-F-G-pNA; Protease Substrate; Chromogenic Peptide Substrate; L-Phenylalanine, N-acetyl-, glycyl-4-nitroanilide
EINECS	Contact for details

Quality Control

Our Ac-Phe-Gly-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided to ensure compliance with research-grade specifications and support your experimental reproducibility.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.