Description

Tetramethylbenzidine (TMB) is a highly sensitive chromogenic substrate widely used in enzymatic assays. Its primary value lies in its ability to produce a distinct, measurable blue color upon oxidation, making it a critical reagent for detection and quantification. This compound is essential for industries and research laboratories requiring precise analytical methods, particularly in life sciences and diagnostics.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): The predominant application, serving as a chromogenic substrate for horseradish peroxidase (HRP) in diagnostic kits for detecting antigens or antibodies.
• Immunohistochemistry (IHC): Used as a staining substrate for HRP-labeled antibodies to visualize specific proteins or antigens in tissue sections.
• Western Blotting: Employed for the chemiluminescent or colorimetric detection of proteins after transfer to a membrane.
• Biosensor Development: Acts as an electron mediator or signal-generating molecule in electrochemical and optical biosensors.
• Clinical Diagnostics: A key component in automated analyzers and point-of-care testing devices for various biomarkers.
• Research & Development: Utilized in biochemical research for studying enzyme kinetics, oxidative processes, and developing new assay methodologies.

Basic Information

Product Name	Tetramethylbenzidine
CAS No.	34314-06-2
Molecular Formula	C16H20N2
Molecular Weight	240.35 g/mol
Synonyms	3,3',5,5'-Tetramethylbenzidine; TMB; TMB Base; 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine; 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl; Benzidine, 3,3',5,5'-tetramethyl-; NSC 406130
EINECS	251-921-2

Quality Control

Our Tetramethylbenzidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive diagnostic and research applications. Certificates of Analysis (COA) detailing purity, absorbance characteristics, and performance in standard HRP assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	168 - 172 °C
Absorbance (in DMSO)	Conforms
Solubility	Soluble in DMSO, DMF; slightly soluble in ethanol
Heavy Metals	< 20 ppm
Loss on Drying	< 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.