Description

Hippuryl-His-Leu CAS NO 31373-65-6 is a synthetic tripeptide substrate specifically designed for enzymatic activity studies. This compound is of critical importance in biochemical research and pharmaceutical development as a selective substrate for angiotensin-converting enzyme (ACE) and other proteases. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in cardiovascular research, enzyme kinetics, and high-throughput screening assays to evaluate inhibitor potency and enzymatic function.

Application

• Angiotensin-Converting Enzyme (ACE) Activity Assays: Primary substrate for the colorimetric or fluorometric determination of ACE activity in research and clinical settings.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel ACE inhibitors for hypertension and heart failure drug development.
• Biochemical Research: Essential tool for studying enzyme kinetics, mechanism of action, and substrate specificity of metalloproteases.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for measuring ACE levels in serum, aiding in the diagnosis of sarcoidosis and other conditions.
• Quality Control in Biologics: Employed to monitor protease activity during the production and purification of therapeutic proteins.
• Academic & Institutional Research: Fundamental reagent for graduate and post-graduate level research in biochemistry, pharmacology, and molecular biology.

Basic Information

Product Name	Hippuryl-His-Leu
CAS No.	31373-65-6
Molecular Formula	C23H30N6O6
Molecular Weight	486.52 g/mol
Synonyms	Hippuryl-L-Histidyl-L-Leucine; N-Benzoylglycyl-L-histidyl-L-leucine; Bz-Gly-His-Leu; ACE Substrate; 2-[[(2S)-1-[[(2S)-1-[[2-(Benzoylamino)acetyl]amino]-3-(1H-imidazol-4-yl)-1-oxopropan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]acetic acid; Hippurylhistidyll leucine; Bz-Gly-His-Leu-OH
EINECS	Contact for details

Quality Control

Our Hippuryl-His-Leu is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Assay	95.0% - 105.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.