Description

Pc Biotin-Peg3-Nhs Ester CAS NO 2353409-93-3 is a heterobifunctional crosslinker featuring a biotin moiety for affinity capture and an NHS ester group for efficient amine coupling. This compound is essential for creating stable, site-specific bioconjugates, enabling high-sensitivity detection and purification in advanced biochemical assays. It is primarily utilized by researchers and manufacturers in the fields of proteomics, antibody-drug conjugate (ADC) development, and diagnostic kit production.

Application

• Site-specific biotinylation of antibodies, proteins, and peptides for immunoassay development.
• Preparation of affinity probes and capture reagents for mass spectrometry (MS) and pull-down assays.
• Conjugation chemistry in the synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutics.
• Functionalization of surfaces and nanoparticles for biosensor and diagnostic device fabrication.
• Labeling of oligonucleotides and other biomolecules for microarray and next-generation sequencing (NGS) applications.
• As a critical reagent in proteomics research for studying protein-protein interactions and post-translational modifications.

Basic Information

Product Name	Pc Biotin-Peg3-Nhs Ester
CAS No.	2353409-93-3
Molecular Formula	C26H41N5O9S
Molecular Weight	599.70 g/mol
Synonyms	Biotin-PEG3-NHS Ester; Biotin-PEG3-NHS; Biotin-(PEG)3-NHS Ester; Biotinamidohexanoyl-PEG3-NHS; 5-((6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)amino)-5-oxopentyl)amino)-5-oxopentyl)biotinamide; PEG3-Biotin-NHS; NHS-PEG3-Biotin; Biotin Polyethylene Glycol NHS Ester (PEG3)
EINECS	Contact for details

Quality Control

Our Pc Biotin-Peg3-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (NHS ester content)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.