Description

n-(m-Peg4)-N'-(Biotin-Peg3)-Cy5 is a sophisticated heterobifunctional fluorescent labeling reagent designed for advanced bioconjugation. This compound is engineered to provide a stable, long-chain PEG spacer for reduced steric hindrance, coupled with biotin for high-affinity capture and the bright, far-red-emitting cyanine dye Cy5 for sensitive detection. It is essential for researchers and developers in the life sciences who require precise, multi-modal tagging of biomolecules. This reagent is particularly valuable for creating probes used in fluorescence imaging, pull-down assays, and diagnostic platform development.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation for in vitro and in vivo imaging, flow cytometry, and microscopy applications.
• Biomolecule Pull-Down and Purification: Utilizes the biotin-streptavidin interaction for affinity-based isolation of tagged complexes.
• Development of Diagnostic Assays: Serves as a key component in ELISA, lateral flow assays, and other biosensors requiring fluorescent readouts.
• Live-Cell and Tissue Imaging Probes: Creation of targeted imaging agents due to the deep tissue penetration of Cy5 fluorescence.
• Surface Functionalization: Modification of nanoparticles, chips, or beads for creating biospecific capture surfaces.
• Drug Delivery System Research: Conjugation to drug carriers for tracking biodistribution and cellular uptake studies.
• Nucleic Acid Labeling: Tagging of oligonucleotides for FISH (Fluorescence In Situ Hybridization) and other genomic techniques.

Basic Information

Item	Detail
Product Name	n-(m-Peg4)-N'-(Biotin-Peg3)-Cy5
CAS No.	2107273-68-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Biotin-PEG3-Cy5-mPEG4; Cy5-mPEG4-Biotin-PEG3; m-PEG4-Biotin-PEG3-Cy5; Biotin-PEG3-Cyanine5-mPEG4; Biotin-(PEG)3-Cy5-(PEG)4; Heterobifunctional PEG Cy5 Biotin Reagent; Biotin-PEG3-Cy5-PEG4-amine; Fluorescent Biotinylation Reagent
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(Biotin-Peg3)-Cy5 is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, mass spectrometry (MS) confirmation, and functional validation of the reactive groups. A detailed Certificate of Analysis (COA) documenting identity, purity, and relevant spectral data is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For optimal performance, solutions should be prepared immediately before use.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (Cy5)	Contact for details
Water Content (KF)	≤ 1.0%
Functional Group Integrity	Conforms (by assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.