Description

n-(Azido-PEG2)-n-Boc-PEG3-NHS Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and an N-hydroxysuccinimide (NHS) ester, separated by a Boc-protected amine. This compound matters for its precise role in bioorthogonal click chemistry and controlled bioconjugation, enabling the site-specific labeling and crosslinking of biomolecules under mild, aqueous conditions. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostics industries for developing antibody-drug conjugates (ADCs), peptide modifications, and advanced hydrogel networks.

Application

• Antibody-Drug Conjugate (ADC) Development: Facilitates the controlled attachment of cytotoxic payloads to monoclonal antibodies via the NHS ester, with the azide handle allowing for subsequent orthogonal modifications or functionalization.
• Peptide and Protein Modification: Used for site-specific PEGylation to improve pharmacokinetics, solubility, and stability of therapeutic peptides and proteins.
• Biomaterial and Hydrogel Synthesis: Serves as a key crosslinking agent in creating functionalized hydrogels for 3D cell culture, tissue engineering, and drug delivery systems.
• Diagnostic Probe Conjugation: Enables the labeling of antibodies, enzymes, or other proteins with fluorescent dyes, enzymes, or other reporter molecules for immunoassays and imaging.
• Surface Functionalization: Used to modify nanoparticles, chips, or other solid supports with bioactive ligands for biosensor and microarray applications.
• Click Chemistry Scaffold: The azide group participates in copper-catalyzed or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions, making it a versatile building block for combinatorial library synthesis.

Basic Information

Product Name	n-(Azido-PEG2)-n-Boc-PEG3-NHS Ester
CAS No.	2093153-85-4
Molecular Formula	C26H45N5O11
Molecular Weight	603.67 g/mol
Synonyms	Azido-PEG2-Boc-PEG3-NHS Ester; Boc-NH-PEG3-NHS Ester Azide; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]-3-[(2-methylpropan-2-yl)oxycarbonylamino]propoxy]ethoxy]ethoxy]propanoate; N-(Azido-PEG2)-N-Boc-PEG3-NHS; Heterobifunctional PEG Crosslinker, Azide-Boc-NHS; NHS-PEG3-Boc-NH-PEG2-Azide; Boc-protected Amine-PEG3-NHS Ester Azide
EINECS	Contact for details

Quality Control

Our n-(Azido-PEG2)-n-Boc-PEG3-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for research and bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical
Functional Group Assay (NHS Ester)	≥ 90% of theoretical

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.