Description

Pc Methyltetrazine-Peg4-Nhs Carbonate Ester is a heterobifunctional crosslinking reagent designed for advanced bioconjugation and labeling applications. This compound is critical for researchers and developers requiring precise, stable linkages between biomolecules and surfaces or probes. It is primarily utilized in the pharmaceutical, diagnostics, and life sciences research sectors for creating antibody-drug conjugates (ADCs), developing biosensors, and functionalizing nanoparticles.

Application

• Antibody-Drug Conjugate (ADC) Development: Enables site-specific conjugation of cytotoxic payloads to monoclonal antibodies via its NHS ester and bioorthogonal tetrazine groups.
• Bioorthogonal Labeling & Imaging: Facilitates live-cell labeling and super-resolution microscopy through rapid, catalyst-free inverse electron-demand Diels-Alder (IEDDA) reactions with trans-cyclooctene (TCO) derivatives.
• Surface Functionalization: Used to modify nanoparticles, microarrays, and biosensor surfaces with bioactive ligands for targeted delivery and detection.
• Protein & Peptide Modification: Allows for the controlled introduction of tetrazine handles onto proteins, peptides, and other amine-containing biomolecules for subsequent click chemistry.
• Polymer & Material Science: Serves as a key building block for synthesizing smart polymers and hydrogels with responsive, bio-clickable properties.
• Diagnostic Probe Synthesis: Essential for constructing fluorescent, radioactive, or magnetic imaging probes for in vitro and in vivo diagnostics.

Basic Information

Product Name	Pc Methyltetrazine-Peg4-Nhs Carbonate Ester
CAS No.	2055736-28-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5-Methyltetrazine-PEG4-NHS carbonate; Methyltetrazine-PEG4-NHS ester; m-Tetrazine-PEG4-NHS; 3-Methyl-1,2,4,5-tetrazine-PEG4-N-hydroxysuccinimide carbonate ester; PC Bioconjugation Reagent; Methyl-Tz-PEG4-NHS; Bioorthogonal Crosslinker 2055736-28-0; Tetrazine NHS-PEG4 carbonate
EINECS	Contact for details

Quality Control

Our Pc Methyltetrazine-Peg4-Nhs Carbonate Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dedicated freezer. Due to its hygroscopic and strictly light-sensitive nature, allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation and photodegradation.

Specification

Item	Specification
Appearance	Red to purple solid or film
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.