Description

Azido-Peg4-Ala-Ala-Asn(Trt)-Pab is a specialized, multifunctional peptide-PEG conjugate designed for advanced bioconjugation and linker applications. This compound matters because it integrates a click-chemistry-ready azide group with a flexible tetraethylene glycol (PEG4) spacer and a protease-cleavable peptide sequence, enabling precise, site-specific modifications of biomolecules under mild conditions. It is primarily needed by researchers and manufacturers in pharmaceutical development, proteomics, and antibody-drug conjugate (ADC) synthesis for constructing targeted therapeutics and diagnostic probes.

Application

• Antibody-Drug Conjugate (ADC) Linker-Payload Construction: Serves as a key component for attaching cytotoxic drugs to antibodies via its cleavable peptide sequence and functional azide handle.
• Site-Specific Protein/Peptide Labeling: Enables controlled modification of biomolecules through copper-free or strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
• Protease-Sensitive Prodrug Development: Used in the design of prodrugs that release active agents upon cleavage by specific enzymes like legumain or cathepsins.
• Biomaterial and Surface Functionalization: Facilitates the creation of bioactive surfaces for cell culture, biosensors, and diagnostic arrays.
• Chemical Biology Tool Development: Acts as a versatile reagent for studying enzyme activity, protein-protein interactions, and intracellular trafficking.
• Polymer and Dendrimer Conjugation: Used to introduce peptide motifs and reactive groups into synthetic polymers for creating smart drug delivery systems.

Basic Information

Product Name	Azido-Peg4-Ala-Ala-Asn(Trt)-Pab
CAS No.	2055042-67-4
Molecular Formula	C58H77N11O13
Molecular Weight	1136.3 g/mol
Synonyms	Azido-PEG4-AAN(Trt)-PAB; N3-PEG4-Ala-Ala-Asn(Trt)-PAB; Azido-PEG4-Ala-Ala-Asn(Trt)-PAB-OH; AAN-PAB(Azido-PEG4); Protease-Cleavable ADC Linker with Azide; 5-((S)-2-((S)-2-((2-(2-(2-(2-Azidoethoxy)ethoxy)ethoxy)ethyl)amino)-3-((S)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3-oxopropyl)propanamido)propanamido)-2-((4-aminobenzyl)oxy)-5-oxopentanoic acid; Fmoc-Asn(Trt)-AAN-PAB(Azido-PEG4)
EINECS	Contact for details

Quality Control

Our Azido-Peg4-Ala-Ala-Asn(Trt)-Pab is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency, meeting the high standards required for research and pre-clinical development. Certificates of Analysis (COA) detailing purity (by HPLC), molecular weight confirmation (MS), and other critical parameters are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.