Description

H-Asn-Pro-Glu-Tyr(Po3H2)-Oh is a chemically synthesized phosphopeptide, a modified amino acid sequence where the tyrosine residue is phosphorylated. This specific post-translational modification is critical for studying and modulating intracellular signaling pathways, particularly those involving protein kinases and phosphatases. It serves as an essential research tool for scientists in biochemistry, molecular biology, and pharmaceutical development who require high-purity, well-characterized peptides for assay development, antibody production, and drug discovery targeting phospho-dependent protein interactions.

Application

• Biochemical Research: Used as a standard or substrate in enzymatic assays to study kinase and phosphatase activity and specificity.
• Antibody Development: Acts as a key antigen for the production and screening of highly specific monoclonal and polyclonal antibodies against phosphorylated tyrosine epitopes.
• Cell Signaling Studies: Employed in pull-down assays, ELISA, and other techniques to investigate protein-protein interactions mediated by phosphotyrosine binding domains (e.g., SH2, PTB domains).
• Pharmaceutical Screening: Serves as a target molecule in high-throughput screening (HTS) campaigns to identify potential inhibitors or modulators of phospho-signaling pathways.
• Diagnostic Development: Utilized in the calibration and validation of diagnostic kits and platforms designed to detect phosphorylation events related to disease states.
• Mass Spectrometry Standard: Provides a reference compound for the identification and quantification of phosphorylated peptides in proteomics research.

Basic Information

Product Name	H-Asn-Pro-Glu-Tyr(Po3H2)-Oh
CAS No.	290810-63-8
Molecular Formula	C₂₀H₂₉N₅O₁₄P
Molecular Weight	603.45 g/mol
Synonyms	Phosphotyrosine Peptide (NPEY); Asparagine-Proline-Glutamic Acid-Phosphotyrosine; NPEY Phosphopeptide; pTyr Peptide; H-Asn-Pro-Glu-pTyr-OH; Phospho-NPEY; NPEpY; L-Asparaginyl-L-prolyl-L-α-glutamyl-O-phosphono-L-tyrosine
EINECS	Contact for details

Quality Control

Our phosphopeptides are manufactured under strict quality management systems. Each batch undergoes comprehensive analytical characterization, including HPLC for purity assessment and MS (Mass Spectrometry) for identity confirmation. We provide Certificates of Analysis (COA) detailing purity, assay, and impurity profiles, ensuring the material meets the stringent requirements for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (HPLC)	≥ 97%
Water Content (KF)	≤ 5.0%
Single Impurity (HPLC)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.