Description

Dabcyl-Arg-Gly-Val-Val-Asn-Ala-Oh is a specialized peptide substrate containing a Dabcyl (4-((4-(dimethylamino)phenyl)azo)benzoic acid) quencher group. This compound is a critical tool in biochemical research, specifically designed for studying protease activity and enzyme kinetics. It is primarily utilized by pharmaceutical researchers, diagnostic developers, and academic laboratories focused on proteomics and drug discovery. The integration of the Dabcyl moiety makes it essential for fluorescence resonance energy transfer (FRET)-based assays.

Application

• Protease Activity Assays: Used as a quenched fluorescent substrate to measure the activity of specific proteases, including caspases and other enzymes involved in apoptosis and cell signaling.
• FRET-Based Research: Serves as a key component in Fluorescence Resonance Energy Transfer (FRET) experiments to study protein-protein interactions and enzymatic cleavage in real-time.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors as therapeutic candidates.
• Biochemical Pathway Analysis: Facilitates the study of proteolytic cascades in disease models, such as cancer, neurodegenerative disorders, and inflammatory conditions.
• Diagnostic Development: Acts as a core reagent in the development of clinical diagnostic kits for detecting abnormal protease levels.
• Enzyme Kinetics Studies: Provides a precise tool for determining kinetic parameters (Km, Vmax, kcat) of target proteases.

Basic Information

Product Name	Dabcyl-Arg-Gly-Val-Val-Asn-Ala-Oh
CAS No.	289898-42-6
Molecular Formula	C48H72N14O12
Molecular Weight	1021.18 g/mol
Synonyms	Dabcyl-RGVVNA-OH; Dabcyl-Arg-Gly-Val-Val-Asn-Ala; Caspase-3 Substrate (Dabcyl-labeled); DABCYL-GRGVVNA; FRET Peptide Substrate; 4-((4-(dimethylamino)phenyl)azo)benzoic acid-Arg-Gly-Val-Val-Asn-Ala; Quenched Fluorescent Peptide; Dabcyl-GRGVVNA-OH
EINECS	Contact for details

Quality Control

Our Dabcyl-Arg-Gly-Val-Val-Asn-Ala-Oh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and MS for identity confirmation, to ensure compliance with research-grade standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.