Description

D-Leucine-15N is a stable isotope-labeled form of the essential amino acid leucine, where the nitrogen atom is enriched with the 15N isotope. This compound is a critical tool for advanced metabolic and proteomic research, enabling precise tracking and quantification of nitrogen incorporation in biological systems. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and nutritional science sectors for applications requiring high isotopic purity and chemical stability.

Application

• Metabolic Pathway Tracing: Used as a tracer in mass spectrometry (MS) and nuclear magnetic resonance (NMR) studies to investigate protein synthesis, turnover, and nitrogen metabolism.
• Quantitative Proteomics: Serves as an internal standard in stable isotope labeling by amino acids in cell culture (SILAC) and other quantitative proteomic methodologies.
• Pharmaceutical Research: Employed in the development and pharmacokinetic studies of peptide-based drugs and diagnostic agents.
• Nutritional Science: Applied in clinical research to study amino acid utilization, protein metabolism, and the impact of dietary interventions.
• Biochemical Reagent: Used in the synthesis of isotopically labeled peptides, standards, and other complex biomolecules for analytical purposes.
• Quality Control Standard: Acts as a certified reference material (CRM) for calibrating analytical instruments in regulatory and quality assurance laboratories.

Basic Information

Item	Detail
Product Name	D-Leucine-15N
CAS No.	287484-39-3
Molecular Formula	C6H1315NO2
Molecular Weight	132.18 g/mol (for 15N labeled form)
Synonyms	(2R)-2-Amino-4-methylpentanoic acid-15N; D-Leucine-15N; (R)-2-Amino-4-methylpentanoic acid-15N; (R)-Leucine-15N; D-2-Amino-4-methylvaleric acid-15N; (R)-2-Amino-4-methylvaleric acid-15N; 15N-D-Leucine; D-Leu-15N
EINECS	Contact for details

Quality Control

Our D-Leucine-15N is manufactured under strict quality management systems. Each batch undergoes comprehensive analysis to ensure high chemical and isotopic purity, meeting the stringent requirements for research and production. Certificates of Analysis (COA) are provided, detailing parameters such as assay (by HPLC), isotopic enrichment (by MS), chiral purity, and residual solvent content. We support compliance with relevant standards for research chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept under dry, inert conditions to maintain stability and purity. For long-term storage, consider desiccated environments.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Isotopic Enrichment (MS)	≥98 atom % 15N
Chiral Purity (HPLC)	≥99.0% D-isomer
Loss on Drying	≤0.5%
Residue on Ignition	≤0.1%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.