Description

Fmoc-[15N]Ala-OH is a stable isotope-labeled derivative of the amino acid alanine, specifically modified with a 9-fluorenylmethoxycarbonyl (Fmoc) protecting group on the amine and a carboxylic acid functional group. This compound is essential for advanced research in peptide synthesis and mass spectrometry, where the 15N label serves as a critical tracer for studying metabolic pathways, protein dynamics, and structural analysis. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and biotechnology companies specializing in proteomics and isotopic labeling.

Application

• Solid-Phase Peptide Synthesis (SPPS): As an Fmoc-protected, isotopically labeled building block for the controlled assembly of peptides.
• Metabolic Flux Analysis: Employed as a 15N tracer to investigate and quantify metabolic pathways in biological systems.
• Proteomics & Mass Spectrometry: Used in the synthesis of internal standards for the accurate quantification of proteins and peptides via LC-MS/MS.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Incorporated into peptides and proteins to simplify complex spectra and facilitate structural elucidation.
• Biomedical Research: Applied in studies of protein-protein interactions, enzyme mechanisms, and drug discovery programs.
• Isotope-Coded Affinity Tag (ICAT) Reagents: A key component in quantitative proteomic techniques for comparative protein expression analysis.

Basic Information

Product Name	Fmoc-[15N]Ala-OH
CAS No.	287484-36-0
Molecular Formula	C18H1715NO4
Molecular Weight	311.33 g/mol (for 15N isotopologue)
Synonyms	Fmoc-L-alanine-15N; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-alanine-15N; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]propanoic-15N acid; Fmoc-Ala(15N)-OH; Fmoc-15N-L-alanine; 9-Fluorenylmethyloxycarbonyl-15N-alanine; Nα-Fmoc-L-alanine-15N
EINECS	Contact for details

Quality Control

Our Fmoc-[15N]Ala-OH is manufactured under strict quality systems to ensure high isotopic and chemical purity for critical research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment, chiral purity, and chemical assay. We adhere to relevant industry standards for fine chemicals and can provide material suitable for use under cGMP guidelines for pharmaceutical development upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (15N)	≥ 98 atom %
Optical Rotation	[α]D20: -20.0° to -25.0° (c=1 in DMF)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.