Description

n-(9-Fluorenylmethoxycarbonyl)-L-Glutamic-15N Acid is a stable isotope-labeled derivative of Fmoc-L-glutamic acid, where the nitrogen atom is enriched with the 15N isotope. This compound is critically important for advanced research applications requiring precise molecular tracking and quantification using techniques like mass spectrometry and NMR. It is primarily needed by pharmaceutical researchers, peptide chemists, and analytical laboratories engaged in proteomics, metabolic pathway studies, and the development of novel peptide-based therapeutics.

Application

• Isotope-Labeled Peptide Synthesis: As a key building block for incorporating a 15N-labeled glutamic acid residue into synthetic peptides, enabling mass spectrometric analysis and quantification.
• Proteomics & Mass Spectrometry (MS): Used as an internal standard for the absolute quantification of proteins and peptides in complex biological samples via techniques like Selected Reaction Monitoring (SRM) or Parallel Reaction Monitoring (PRM).
• Metabolic Pathway Tracing: Employed in metabolic flux analysis studies to track the incorporation of nitrogen in glutamic acid and related metabolites within biological systems.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Serves as a valuable tool in structural biology and mechanistic studies, where the 15N label provides a sensitive probe for investigating molecular structure and dynamics.
• Pharmaceutical R&D: Utilized in the research and development of peptide drugs, where labeled analogs are essential for studying absorption, distribution, metabolism, and excretion (ADME) properties.
• Bioconjugation & Chemical Biology: Acts as a precursor for creating labeled probes and linkers for studying protein-protein interactions and enzyme activities.

Basic Information

Product Name	n-(9-Fluorenylmethoxycarbonyl)-L-Glutamic-15N Acid
CAS No.	287484-34-8
Molecular Formula	C₂₀H₁₉¹⁵NNO₆
Molecular Weight	Contact for details
Synonyms	Fmoc-L-Glu(15N)-OH; Fmoc-L-glutamic-15N acid; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-glutamic-15N acid; (2S)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl]amino]pentanedioic-15N acid; 15N-Fmoc-Glu-OH; Fmoc-Glu(15N)-OH; Nα-Fmoc-L-glutamic-15N acid
EINECS	Contact for details

Quality Control

Our n-(9-Fluorenylmethoxycarbonyl)-L-Glutamic-15N Acid is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, chemical purity, and isotopic enrichment. A comprehensive Certificate of Analysis (COA) detailing assay, isotopic purity, and impurity profile is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (15N)	≥ 98 atom %
Chemical Purity	≥ 95.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.