Description

L-Methionine-1-13C n-T Boc Derivative is a stable isotope-labeled, N-terminal tert-butoxycarbonyl (Boc) protected derivative of the essential amino acid methionine. This compound is a critical building block for the synthesis of isotopically labeled peptides and proteins, enabling advanced research in metabolic studies, proteomics, and pharmacokinetics. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and CROs (Contract Research Organizations) engaged in tracer studies and mass spectrometry-based analytical method development.

Application

• Isotope-Labeled Peptide Synthesis: Serves as a key precursor for incorporating carbon-13 labeled methionine into custom peptides for mass spectrometry quantification and protein turnover studies.
• Metabolic Pathway Tracing: Used in 13C metabolic flux analysis to track the incorporation of methionine into proteins and other biomolecules within living systems.
• Proteomics Research: Enables the development of internal standards for absolute quantification (AQUA) in targeted proteomics using techniques like LC-MS/MS.
• Pharmaceutical Development: Employed in the research and development of novel therapeutics, particularly in studying drug metabolism and protein-drug interactions.
• Biochemical Research: Acts as a specialized reagent for studying methionine metabolism, methylation processes, and related biochemical pathways.
• Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Can be used in specialized SILAC protocols for precise proteomic profiling.

Basic Information

Product Name	L-Methionine-1-13C n-T Boc Derivative
CAS No.	286437-20-5
Molecular Formula	C10H1913CNO4S
Molecular Weight	279.78 g/mol (for 13C1 isotopologue)
Synonyms	(2S)-2-[(tert-Butoxycarbonyl)amino]-4-(methylsulfanyl)butanoic-1-13C acid; N-(tert-Butoxycarbonyl)-L-methionine-1-13C; Boc-L-Met-1-13C; N-Boc-L-Methionine-1-13C; tert-Butyl (2S)-2-amino-4-(methylthio)-1-13C-butanoate; Boc-Met-1-13C-OH; L-Methionine, N-[(1,1-dimethylethoxy)carbonyl]-, 1-13C
EINECS	Contact for details

Quality Control

Our L-Methionine-1-13C n-T Boc Derivative is manufactured under strict quality control protocols to ensure high chemical purity and isotopic enrichment. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry to confirm identity, chemical purity, and isotopic purity. A comprehensive Certificate of Analysis (COA) detailing assay, isotopic enrichment, chiral purity, and impurity profile is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Enrichment (13C)	≥ 99 atom % 13C
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.