Description

(2S,4R)-1-(((9H-Fluoren-9-Yl)Methoxy)Carbonyl)-4-((tert-Butoxycarbonyl)Amino)Pyrrolidine-2-Carboxylic Acid CAS NO 273222-06-3 is a high-purity, protected amino acid derivative, specifically an Fmoc/Boc-protected proline analog, essential for advanced peptide synthesis. This compound is critical for researchers and manufacturers requiring precise stereochemical control and orthogonal protecting group strategies in complex molecule assembly. It is primarily utilized by the pharmaceutical, biotechnology, and academic research sectors for developing novel therapeutic peptides, peptidomimetics, and as a key chiral building block in medicinal chemistry.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a key Fmoc-protected building block for the controlled incorporation of (2S,4R)-4-aminoproline into peptide chains.
• Medicinal Chemistry & Drug Discovery: Serves as a crucial intermediate in the synthesis of peptide-based drug candidates, particularly those targeting protein-protein interactions.
• Peptidomimetic Development: Used to create conformationally constrained analogs to enhance metabolic stability, bioavailability, and receptor selectivity of lead compounds.
• Bioconjugation & Chemical Biology: Employed in the synthesis of labeled peptides or peptide-drug conjugates for diagnostic and therapeutic applications.
• Academic & Process R&D: A vital reagent for methodological development in organic synthesis and for scaling up novel peptide sequences.
• API Intermediate Manufacturing: Acts as a defined starting material or intermediate in the regulated synthesis of Active Pharmaceutical Ingredients (APIs).

Basic Information

Item	Details
Product Name	(2S,4R)-1-(((9H-Fluoren-9-Yl)Methoxy)Carbonyl)-4-((tert-Butoxycarbonyl)Amino)Pyrrolidine-2-Carboxylic Acid
CAS No.	273222-06-3
Molecular Formula	C25H28N2O6
Molecular Weight	452.50 g/mol
Synonyms	Fmoc-(2S,4R)-4-(Boc-amino)proline; (2S,4R)-Fmoc-4-(Boc-NH)-Pro-OH; Fmoc-(2S,4R)-4-[(tert-Butoxycarbonyl)amino]proline; (2S,4R)-1-(9-Fluorenylmethoxycarbonyl)-4-[(tert-butoxycarbonyl)amino]pyrrolidine-2-carboxylic acid; (2S,4R)-4-[(tert-Butoxycarbonyl)amino]-1-[(9H-fluoren-9-ylmethoxy)carbonyl]pyrrolidine-2-carboxylic acid; Boc-4-Amino-(2S,4R)-Pro-OH-Fmoc; Fmoc-Aze(Boc)-OH (for azetidine-2-carboxylic acid derivative); Protected cis-4-Aminoproline derivative
EINECS	Contact for details

Quality Control

Our production of (2S,4R)-1-(((9H-Fluoren-9-Yl)Methoxy)Carbonyl)-4-((tert-Butoxycarbonyl)Amino)Pyrrolidine-2-Carboxylic Acid adheres to stringent quality protocols suitable for research and cGMP-grade applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, chiral integrity, and impurity profiles. We ensure compliance with relevant ICH guidelines and can provide material for regulatory filings. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥98.0%
Chiral Purity (HPLC)	≥99.0% de (2S,4R)
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.