Description

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-Nh2 is a synthetic, fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is of significant value for researchers and developers in the life sciences sector, particularly for studying protease activity and enzyme kinetics. It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic biochemistry laboratories focused on proteolytic pathways and inhibitor screening.

Application

• Protease Activity Assays: As a specialized fluorogenic substrate for the detection and quantification of specific protease enzymes.
• Enzyme Kinetic Studies: Used to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) for target proteases.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize novel protease inhibitors.
• Diagnostic Development: Serves as a key component in the development of clinical diagnostic tests for diseases involving dysregulated protease activity.
• Biochemical Research: A critical tool for fundamental research into proteolytic signaling cascades and protein degradation mechanisms.

Basic Information

Product Name	Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-Nh2
CAS No.	256394-92-0
Molecular Formula	C48H70N14O15
Molecular Weight	1051.2 g/mol
Synonyms	7-Methoxycoumarin-4-acetyl-Pro-Leu-Ala-Nva-Asp(Dnp)-Ala-Arg-NH2; Mca-PLANva-Dap(Dnp)-AR-NH2; Fluorogenic Peptide Substrate Mca-PLANva-Dap(Dnp)-AR-NH2; Caspase-3 Substrate II (fluorogenic); Ac-DEVD-AFC Alternative; (7-Methoxycoumarin-4-yl)acetyl-Pro-Leu-Ala-Nva-Asp(2,4-dinitrophenyl)-Ala-Arg-amide
EINECS	Contact for details

Quality Control

Our Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-Nh2 is manufactured under strict quality protocols suitable for research and in-vitro diagnostic use. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, assay results, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.